Thanks so much for the comment! Be sure to watch my next video. Testing species in meats (e.g beef, pork, chicken, sheep etc). It took a lot of time and resources to get this! :) The kitty is all grown up and freaking FAT now! :(((
I am happy if you find my videos useful somehow! Best of luck with the grapes. I’m trying to do a gene therapy. :) p.S check out the group Petalsmyths Plant Engineering Research Party on facebook. Those guys are also in plant bioengineering (independent researchers).
Thanks for the comment. U can also watch my gene therapy project update. Just finished 2 of the mammalian plasmid vectors and I will post an update very soon!
Don't mention it. Thanks for the comment. Also, if you've followed my other videos I use snapgene as my go to program for my gene therapy project. I kinda simulate basically everything. From pcr, gel to golden gate assembly.
Is it possible to do the cloning without using restriction enzymes here? As in I want to insert a gene by replacing a specific in order to decide the size of amplicon.
I don't know if it's possible to simulate in snap gene. For non-enzime cloning I would suggest PIPE assembly. bitesizebio.com/23889/polymerase-incomplete-primer-extension-pipe-cloning-method/. David Ishee has an awesome video on this :)
With the primer aren’t you getting a sequence of dna that is not the gene in the new plasmid? Can you just add restriction sites and primers that introduce new bases in vectors that will be introduced in a genome?
Yeah u can. I do this all the time. In the portion that does not anneal i put restriction sites. For example u can check my Golden Gate Assembly video. I’ve recently published a paper in which i built 21 plasmids from scratch. I have all the primers used there in additional info.
Hello Rakesh! Thank you so much for your feedback! I will try my best to make these videos. Until then, I would highly suggest an alternative to Gibson. It's called Golden Gate. I use a slight modification of it and I do have a video explaining in detail how I do it. th-cam.com/video/9b1V2HKhg5A/w-d-xo.html. I have successfully assembled like 20 plasmids using this. If you want to try it out I can help you a bit with some infos. Add me on facebook...Adrian Ionut Pascu. I don't really use gibson on TA cloning to be honest. For simple cloning of one or two fragments I would also suggest P.I.P.E assembly (it makes use of the imperfection of PCR) Polymerase Incomplete Primer Extension. Here is a link for it. David Ishee. odysee.com/@DavidIshee:d/dna-pipe-assembly-method:4 . Hope this helps :)
Genuinely, one of the most helpful videos I've ever seen. Thank you so much.
Instantly subscribed, and am excited to view your other videos.
Thank you so much! I would like to do an update video on my research soon. 🙏🏼
Amazing video!! you just saved my cloning project!!!
(Also cute cat)
Thanks so much for the comment! Be sure to watch my next video. Testing species in meats (e.g beef, pork, chicken, sheep etc). It took a lot of time and resources to get this! :) The kitty is all grown up and freaking FAT now! :(((
That's sooo helpful! Continue this amazing work:)
Thank you for the comment! 🙏 make sure to subscribe. Videos will be coming soon. Also, don’t miss the giveaways.
It was a very informative and easy-to-understand video. Thank you!
Highly appreciate the comment! Thanks!
Mr Adrian I Pascu I am working breeding programm of seedless grapes and your skills will increase grape export potential in Central Asia! Thank a lot
I am happy if you find my videos useful somehow! Best of luck with the grapes. I’m trying to do a gene therapy. :) p.S check out the group Petalsmyths Plant Engineering Research Party on facebook. Those guys are also in plant bioengineering (independent researchers).
Seedless grapes are not good ,BCz for next grape plant it can create problem ,BCz no seed available😅 for next progeny😊
Awesome video! Finally someone who explains every small detail. Thank you sir!
Thank you so much!
Love it! And your cat!
Thanks for the comment. U can also watch my gene therapy project update. Just finished 2 of the mammalian plasmid vectors and I will post an update very soon!
Very helpful!! Thank you!!
Thanks for the comment! Appreciate it! Check out my other videos. You might find those useful too! Have a nice day! 🙏🏼
OMG thanks a lot for your job you've helped me so much THANK YOU
Don't mention it. Thanks for the comment. Also, if you've followed my other videos I use snapgene as my go to program for my gene therapy project. I kinda simulate basically everything. From pcr, gel to golden gate assembly.
Is it possible to do the cloning without using restriction enzymes here? As in I want to insert a gene by replacing a specific in order to decide the size of amplicon.
I don't know if it's possible to simulate in snap gene. For non-enzime cloning I would suggest PIPE assembly. bitesizebio.com/23889/polymerase-incomplete-primer-extension-pipe-cloning-method/. David Ishee has an awesome video on this :)
Wonderful !!
Thank you so much for the feedback!
With the primer aren’t you getting a sequence of dna that is not the gene in the new plasmid? Can you just add restriction sites and primers that introduce new bases in vectors that will be introduced in a genome?
Yeah u can. I do this all the time. In the portion that does not anneal i put restriction sites. For example u can check my Golden Gate Assembly video. I’ve recently published a paper in which i built 21 plasmids from scratch. I have all the primers used there in additional info.
please make video on gibson ,topo ta cloning ..how to delete gene..please sir..please..really helpful for me..awesome video.full of knowledge
Hello Rakesh! Thank you so much for your feedback! I will try my best to make these videos. Until then, I would highly suggest an alternative to Gibson. It's called Golden Gate. I use a slight modification of it and I do have a video explaining in detail how I do it. th-cam.com/video/9b1V2HKhg5A/w-d-xo.html. I have successfully assembled like 20 plasmids using this. If you want to try it out I can help you a bit with some infos. Add me on facebook...Adrian Ionut Pascu. I don't really use gibson on TA cloning to be honest. For simple cloning of one or two fragments I would also suggest P.I.P.E assembly (it makes use of the imperfection of PCR) Polymerase Incomplete Primer Extension. Here is a link for it. David Ishee. odysee.com/@DavidIshee:d/dna-pipe-assembly-method:4 . Hope this helps :)