Nicolas, many thanks for this webinar. One question: when using a 10 colors "traditional" instrument, and running a 5colors panel, is it better to 1) acquire with all PMTs opened and use spectral compensation or 2) close unused PMTs and go for traditional or Autospill compensation? Thanks, Vincent
Hi Vincent! Based on our testing (presented in this video), using option 1 (acquire with all PMTs opened and use spectral compensation) will give you less spread of your data than option 2 (using non spectral comp). So I you have spreading issue with your data that cannot be resolved with better panel design, I will recommend you to acquire your data with all PMTs opened and use spectral compensation. Best
Hello again, here at th-cam.com/video/5_AB-qVEZqs/w-d-xo.html, for Positive, you use -for ex- "Size/APC-H7-A+", so you need to adjust the "APC-H7-A+" gate or is it ok to use "only" as positive the "Size" (or Clean up) gate? Thanks
Nicolas, many thanks for this webinar. One question: when using a 10 colors "traditional" instrument, and running a 5colors panel, is it better to 1) acquire with all PMTs opened and use spectral compensation or 2) close unused PMTs and go for traditional or Autospill compensation? Thanks, Vincent
Hi Vincent! Based on our testing (presented in this video), using option 1 (acquire with all PMTs opened and use spectral compensation) will give you less spread of your data than option 2 (using non spectral comp).
So I you have spreading issue with your data that cannot be resolved with better panel design, I will recommend you to acquire your data with all PMTs opened and use spectral compensation.
Best
@@NicolasLoof Dear Nicolas, thanks for your reply.
Hello again, here at th-cam.com/video/5_AB-qVEZqs/w-d-xo.html, for Positive, you use -for ex- "Size/APC-H7-A+", so you need to adjust the "APC-H7-A+" gate or is it ok to use "only" as positive the "Size" (or Clean up) gate? Thanks