Using the BufferPro function in ӒKTA avant 25 for automated preparation of buffer plates

Preparing buffers manually for buffer conditions screening might be very time consuming. Using ÄKTA avant system will significantly reduce manual work and add control with in-line pH and conductivity recording during buffer preparation. This videos shows how to use the BufferPro function in ÄKTA avant for automated preparation of buffer plates.
0:00 Introduction
0:42 Import the downloaded methods in Method Editor
1:11 In UNICORN software, set fraction collection parameters in System Control
1:26 Open the method for second buffer system in Method Editor
1:47 Set pH and % B buffer (salt)
2:03 Enter settings for relevant plates or tubes
2:13 Prepare flow path and buffers for second buffer system
2:24 Prepare fraction collector
2:35 Create Method Queue to enable filling of multiple plates for the second buffer system
3:27 Cover filled wells
3:38 Second buffer system finished
3:43 Open the method for first buffer system in Method Editor
3:51 Select buffer system
4:27 Prepare flow path and buffers for first buffer system
4:31 Create Method Queue for the first buffer system
5:01 Start the Method Queue in System Control
5:21 You can now start your screening experiment
5:32 Edit fraction settings in System Control
5:47 Edit buffer recipe in Method Editor
6:31 Edit Fractube type and Frac volume
6:43 Edit scouting settings