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Iowa Institute of Human Genetics
United States
เข้าร่วมเมื่อ 16 พ.ค. 2014
Growth in human genetics research is driving the increasing use of genetics in medical practice. The environment of the Iowa Institute of Human Genetics (IIHG) provides unique opportunities to make progress in both the discovery and translational phases of human genetics. The IIHG is a statewide resource that promotes clinical care, research, and education in human genetics and is focused on bringing personalized genomic medicine to the state of Iowa.
Exploring Single Cell Analysis with Loupe Browser
Exploring Single Cell Analysis with Loupe Browser
มุมมอง: 336
วีดีโอ
Tips for Shadowing a Genetic Counselor
มุมมอง 1445 หลายเดือนก่อน
Helpful tips to ensure you get the most out of your shadowing experience.
University of Iowa and 10X Genomics Single Cell Symposium - March 21, 2024
มุมมอง 1898 หลายเดือนก่อน
University of Iowa and 10X Genomics Single Cell Symposium - March 21, 2024
Customizing single cell applications to interrogate mechanisms of acquired drug resistance in cancer
มุมมอง 6082 ปีที่แล้ว
For the third talk in the IIHG Computational Biology Seminar Series, we are pleased to welcome Dr. Adam Dupuy, Associate Professor of Anatomy and Cell Biology in the Carver College of Medicine, University of Iowa. Adam is a cancer geneticist who works with the Sleeping Beauty transposon system to perform phenotype-selected forward genetic screens, focusing on cancer and drug resistance. His lab...
Visualization of RNA Sequencing Data with PCA clustering and Heatmaps in RR Studio clean
มุมมอง 11K3 ปีที่แล้ว
As sequencing technologies continue to improve and assessment of the transcriptome with RNA-Sequencing becomes more commonplace, it is important that the proper methods are in place to analyze the large amounts of data. R/R Studio contains many packages that are useful for both statistical analysis and visualization of large datasets. In this webinar, we will focus specifically on two of the mo...
Introduction to Bioconductor and Public Genomic Data in R
มุมมอง 9K3 ปีที่แล้ว
An online workshop of the IIHG Bioinformatics Division presented by Jason Ratcliff, MS. Topics covered include Bioconductor and NCBI GEO, and using programmatic methods to access data. Goals of this workshop include introducing common R/Bioconductor data structures, how to work with data in R, a brief description of Object Oriented programming and combining these ideas to apply to accessing pub...
NGS Interest Group Beyond the DE gene list understanding your RNA seq results
มุมมอง 6573 ปีที่แล้ว
RNA-seq has quickly taken over as the preeminent experiment for measuring gene expression in tissue samples. The technique has a wide array of applications from understanding the role of a gene knockout to assessing the impact of a novel drug. While most investigators are focused on the differentially expressed (DE) genes detected in the experiment, there is a wealth of other information that c...
NGS-10x Genomics Sample Prep for Chromium Single Cell Gene Expression, ATAC, and Multiome Solutions
มุมมอง 10K3 ปีที่แล้ว
First, we will provide an overview of 10x Genomics Chromium and Visium solutions. Next, we will cover general sample preparation guidelines for Chromium single cell solutions, including important concepts when isolating cells or nuclei from mouse embryonic and adult brains. Finally, we will review how to isolate nuclei for single cell gene expression, ATAC, and multiome ATAC gene expression sol...
Next Generation Sequencing Interest Group - Considerations for RNA-Seq and Multi-omics Experiments
มุมมอง 6233 ปีที่แล้ว
Next Generation Sequencing Interest Group - Considerations for RNA-Seq and Multi-omics Experiments
Genomic Analysis of Chronic Staphylococcus Aureus Infections in Cystic Fibrosis
มุมมอง 6843 ปีที่แล้ว
This talk was given by Anthony Fischer, MD, PhD, Assistant Professor of Pediatrics (Pulmonary Medicine, UIHC) on Feb 23, 2021. Dr. Fischer is discussing using whole genome sequencing to analyze isolates of Staphylococcus aureus cultured from patients with cystic fibrosis. Through this work, Dr. Fischer has learned that children and adults with cystic fibrosis are often infected with different s...
Introduction to ACMG Genetic Variant Classification
มุมมอง 8K4 ปีที่แล้ว
The American College of Medical Genetics (ACMG) has comprehensive but complex guidelines on how to classify human genetic variants, from benign to pathogenic. Learn how to use and apply these guidelines in your research or clinical testing, including common mistakes and how to adjust criteria strength. Speaker: Diane Kolbe, PhD. Iowa IIHG: medicine.uiowa.edu/humangenetics/ Iowa MORL: morl.lab.u...
Genetic Counseling Interns Do's and Don'ts, 2019
มุมมอง 2.2K5 ปีที่แล้ว
A short video describing the "Do's" and "Don'ts" of a genetic counseling session. Brought to you by the 2019 Intern class of the IIHG Genetic Counseling program. To learn more, visit: medicine.uiowa.edu/humangenetics/education medicine.uiowa.edu/humangenetics/links-interest www.aboutgeneticcounselors.com/ www.nsgc.org/
Introduction to Metagenomics for Researchers
มุมมอง 49K5 ปีที่แล้ว
In this screencast, I discuss why we should care about microbiomes and what is metagenomics more generally. I also talk about computational methods for analysis, and look at the nature of metagenomics data and how it differs from other types of data. Many of the slides in this talk are adapted from slides prepared by Curtis Huttenhower at Harvard (huttenhower.sph.harvard.edu/) and are re-used h...
10X Genomics Loupe Single Cell Browser Explained in 7 minutes
มุมมอง 20K6 ปีที่แล้ว
This video is a short introduction to the 10X Genomics desktop single-cell browser software called "Loupe Cell Browser." In 7 minutes, I attempt to explain the basic features of the software and how you can use it to quickly get a view onto your single-cell data. We in the Iowa Institute of Human Genetics Bioinformatics Division have the pipelines and expertise necessary to help you process and...
Introduction to RNA-Seq for Researchers
มุมมอง 99K7 ปีที่แล้ว
Introduction to RNA-Seq for Researchers
RNA-seq results explained: what you can expect from an analysis
มุมมอง 9K7 ปีที่แล้ว
RNA-seq results explained: what you can expect from an analysis
Quick Start Guide to Running Ingenuity Pathway Analysis (IPA)
มุมมอง 28K7 ปีที่แล้ว
Quick Start Guide to Running Ingenuity Pathway Analysis (IPA)
IIHG Intro to the UCSC Genome Browser | Part 5 of 5
มุมมอง 1.2K8 ปีที่แล้ว
IIHG Intro to the UCSC Genome Browser | Part 5 of 5
IIHG Intro to the UCSC Genome Browser | Part 3 of 5
มุมมอง 2.3K8 ปีที่แล้ว
IIHG Intro to the UCSC Genome Browser | Part 3 of 5
IIHG Intro to the UCSC Genome Browser | Part 4 of 5
มุมมอง 1.5K8 ปีที่แล้ว
IIHG Intro to the UCSC Genome Browser | Part 4 of 5
IIHG Intro to the UCSC Genome Browser | Part 2 of 5
มุมมอง 3.9K8 ปีที่แล้ว
IIHG Intro to the UCSC Genome Browser | Part 2 of 5
IIHG Intro to the UCSC Genome Browser | Part 1 of 5
มุมมอง 7K8 ปีที่แล้ว
IIHG Intro to the UCSC Genome Browser | Part 1 of 5
we need as a bioinformatices, more tutorials about the.. run double finder structures such as (seu,PCs , pN etc ..) and how to control them and when ??? Please help .. tanx
the trick is, how to Annotate clusters from integrated databases ?
Hello, I would like to thank you so much for making this video, I have spent so long looking for one which gives a simple step by step explanation of how to make a customized PCA plot with the DESeq data I have and this worked perfectly. Thank you.
😮😮😮😮
May i ask, how exactly do you find these universal primers? Do we just type 16s in NCBI, then find the primer for that? Because usually, in NCBI, they're species specific, not umiversal
Awesome!
I am currently studying Wilson Disease, and based on the literature I have gathered, there is no well-established genotype-phenotype association. Also, there are multiple mutations across all 21 exons of the ATP7B gene. But most of the literature also state that they are using ACMG guidelines for classifying variants. Any comment on that?
Very good presentation. thanks for sharing.
Awesome, thanks a lot for this video
Thank you Iowa Institute for Human Genetics. Greetings from a bioeng grad student from Universidad del Cauca, Colombia.
Thank you very much for this wonderfully presented material.
Great video. Thank you!
I don't understand. If you want to detect what genes are off or on and by how much they are expressed then why not just use epigenomics instead of transcriptomics?
lache la traduction en français ta voix de cancéreux là
Best lecture on metagenomics, wish for further information 👌
Could you please share the link
Wish I could see this great video earlier. So many questions solved.
great webinar!
Is this just applied to gremlin variant? Can it be applied to somatic variant?
Thank you 10x team and the host. Can I get a copy of the slides to use for my rehearsals? I am trying to understand the work flow of single cell seq to use on my postgraduate project and this could be of great help. Again, thank you so much for the presentation.
Hello, awesome video. Could the .html be shared? thanks
Could you please give us access to the document used in the video?
I have chronic gut issues that comes along with all other kinds of side effects. Is there any way to get into this study as a patient? I have an appointment at Ohio state coming soon. My life is falling apart and most of my doctors don't seem to understand the severity of it.
This is such a helpful video. Thank you so much!
Could you please give link to access data shown in video
This was excellent -- are slides publicly available as implied at the end of the lecture?
Lauren, I don't believe they are at this time. You could email Diana directly and ask for a copy.
thank you so much for the presentation! well explained!
Glad it was helpful!
thanks
Dr. Michael mentions at 48:17 that he did a webinar on pathway analysis. Could you please share the link? Thank you!
This video is a great summary of Metagenomics!
Thanks so much for the video. Very informative. Uts my first time to read about metagenomics because I have an interest for my PhD and your video motivates me. Thanks 😊
You're very welcome!
Thanks for the video. Please make a video on chip seq analysis in R starting with GEO dataset, there is no proper one on internet so far.
Great idea...we'll try to make that happen!
Nice video, thanks Michael! I'm a molecular biologist who struggles with the basics of bioinformatics so this really helped.
Great to hear!
Thank you very much Dr. Kolbe this is a very useful and informative overview.
You are very welcome
Thanks a lot. very well explained.
Glad it was helpful!
This is wonderful information about bioconductor and Public database!
thanks for sharing, greetings from Spain!
Thanks for watching!
theng you...
This is really terrific, thank you so much for providing it to all. The 10X folks did a great job IMHO: very well organized and clear, many tips on how to succeed. Hoping to run my first 10X experiment very soon and this is very reassuring information to have at hand. Hopefully I can find a pdf of these presentations, but if not I'll just take screen captures. Cheers!
Informative and concise. Thanks!
Great video! Thank you!
Really appreciate this explanation, as I'm having my first go at writing a protocol for a job.
this is amazing helpful! wow thank you 10x !!
Great presentation! Systematic and very easy to follow, thanks for putting this together!
Glad it was helpful!
BA1 is benign-standalone not benign-automatic. Its weight is so heavy that just that criteria itself can cause the variant to be considered benign as the name implies.
Yes, the formal name is benign stand-alone. In practice, unless dealing with a well-known and established high-frequency pathogenic variant such as the global exception list, if BA1 is satisfied, the variant can be automatically considered benign without considering other criteria.
Excellent!
Many thanks!
Thanks. Very well-described
Glad it was helpful!
Great video! Cheers
Thanks!
Great! So explanatory. Thank you.
Glad it was helpful!
Thank you