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DR MAKKY
United States
เข้าร่วมเมื่อ 7 พ.ค. 2020
This's an educational channel that provides simple scientific knowledge for understanding.
Dr. Makky EA
Currently, he is Associate Professor at the Faculty of Industrial Sciences & Technology (FIST), Universiti Malaysia Pahang (UMP), Malaysia. Head of Green Technology Research Cluster (2015-2017), where he obtained Ph.D. 2005 in Applied Microbiology, Master degree in Microbial Enzymes Biotechnology and got Post-doctoral Fellow from University of Seoul (UOS), South Korea. His field of interests includes Applied Microbiology, Medical Microbiology, Waste Management, Biotechnology, Bioremediation, Microbial Physiology, Microbial enzymes, and Microbial Nanotechnology.
Dr. Makky EA
Currently, he is Associate Professor at the Faculty of Industrial Sciences & Technology (FIST), Universiti Malaysia Pahang (UMP), Malaysia. Head of Green Technology Research Cluster (2015-2017), where he obtained Ph.D. 2005 in Applied Microbiology, Master degree in Microbial Enzymes Biotechnology and got Post-doctoral Fellow from University of Seoul (UOS), South Korea. His field of interests includes Applied Microbiology, Medical Microbiology, Waste Management, Biotechnology, Bioremediation, Microbial Physiology, Microbial enzymes, and Microbial Nanotechnology.
Streptococcus pyogenes: Characteristics, Classification, Antigenic structure, Toxins & Enzymes
#DRMAKKY #microbiology #microbiologystudent #microbiologylife #immunesystem #lifescienceskills
This video explains Streptococcus pyogenes Characteristics, Classification, Antigenic structure, Toxins & Enzymes. These bacteria are facultative anaerobic, G+ve coccus, non-motile, non-spore-forming, catalase (-ve) which grows in chains and causes infections in humans including pharyngitis, tonsillitis, scarlet fever, cellulitis, rheumatic fever, myonecrosis and lymphangitis. The only known reservoirs for Group A Streptococcus (GAS) in nature are the skin and mucous membranes of the human host. The cell wall of S. pyogenes consists of the following components: Capsular hyaluronic acid: Non-antigenic and has weak anti-phagocytic activity but protect streptococci against immunological attacks. Cell-wall: 1. Outer layer: Protein and lipoteichoic acid. 2. Middle layer: Group specific carbohydrates. 3. Inner layer: Peptidoglycan (mucoprotein), responsible for cell wall rigidity, and enhances non-specific resistance (pyogenic and thrombolytic activity). Group A-specific carbohydrates antigens (C-Ags): Serological (Lancefield) grouping of streptococci is done and divided into several groups (A to V). All streptococci except viridans (α-hemolytic) group has a carbohydrate layer.
Proteins: present in outer layer, produce surface protein antigens (F, M, T, and R), and useful in serological typing of S. pyogenes. F-protein: recognizes host fibronectin (a protein present in eukaryotic cells), and helps in attachment lipoteichoic acid and M-protein together. M-protein: Antigenic, acts as a virulence factor by inhibiting phagocytosis, heat, and acid-stable but susceptible to tryptic digestion, and covered with lipoteichoic acid that enables the organism to attach to epithelial cells. T-protein: common to many M-types, strongly antigenic, not associated with virulence, and not protective Ag. R-protein: strongly antigenic, non-type specific, and associated with some M-protein types called M-associated protein (MAP), not associated with virulence and not protective Ag. Pili (Fimbriae): Hair-like and project from the capsule, consists of partly M-protein, covered with lipoteichoic acid, important in the attachment of streptococci to epithelial cells.
th-cam.com/users/DRMAKKY
drmakky101902.soci.vip/
#Facebook
DR-MAKKY-102340904845636/?view_public_for=102340904845636
www.tubebuddy.com/ykee7/u3#sec1
Image sources
www.brainkart.com/article/Cell-Wall-Components-and-Antigenic-Structure---Streptococcus-pyogenes_18018/
quizlet.com/161554833/micro-quiz-14-streptococcus-pyogenes-flash-cards/
www.bacteria.cz/streptococcus-pyogenes-infections.html
microbiologynotes.com/differences-between-flagella-and-pili/
This video explains Streptococcus pyogenes Characteristics, Classification, Antigenic structure, Toxins & Enzymes. These bacteria are facultative anaerobic, G+ve coccus, non-motile, non-spore-forming, catalase (-ve) which grows in chains and causes infections in humans including pharyngitis, tonsillitis, scarlet fever, cellulitis, rheumatic fever, myonecrosis and lymphangitis. The only known reservoirs for Group A Streptococcus (GAS) in nature are the skin and mucous membranes of the human host. The cell wall of S. pyogenes consists of the following components: Capsular hyaluronic acid: Non-antigenic and has weak anti-phagocytic activity but protect streptococci against immunological attacks. Cell-wall: 1. Outer layer: Protein and lipoteichoic acid. 2. Middle layer: Group specific carbohydrates. 3. Inner layer: Peptidoglycan (mucoprotein), responsible for cell wall rigidity, and enhances non-specific resistance (pyogenic and thrombolytic activity). Group A-specific carbohydrates antigens (C-Ags): Serological (Lancefield) grouping of streptococci is done and divided into several groups (A to V). All streptococci except viridans (α-hemolytic) group has a carbohydrate layer.
Proteins: present in outer layer, produce surface protein antigens (F, M, T, and R), and useful in serological typing of S. pyogenes. F-protein: recognizes host fibronectin (a protein present in eukaryotic cells), and helps in attachment lipoteichoic acid and M-protein together. M-protein: Antigenic, acts as a virulence factor by inhibiting phagocytosis, heat, and acid-stable but susceptible to tryptic digestion, and covered with lipoteichoic acid that enables the organism to attach to epithelial cells. T-protein: common to many M-types, strongly antigenic, not associated with virulence, and not protective Ag. R-protein: strongly antigenic, non-type specific, and associated with some M-protein types called M-associated protein (MAP), not associated with virulence and not protective Ag. Pili (Fimbriae): Hair-like and project from the capsule, consists of partly M-protein, covered with lipoteichoic acid, important in the attachment of streptococci to epithelial cells.
th-cam.com/users/DRMAKKY
drmakky101902.soci.vip/
DR-MAKKY-102340904845636/?view_public_for=102340904845636
www.tubebuddy.com/ykee7/u3#sec1
Image sources
www.brainkart.com/article/Cell-Wall-Components-and-Antigenic-Structure---Streptococcus-pyogenes_18018/
quizlet.com/161554833/micro-quiz-14-streptococcus-pyogenes-flash-cards/
www.bacteria.cz/streptococcus-pyogenes-infections.html
microbiologynotes.com/differences-between-flagella-and-pili/
มุมมอง: 1 235
วีดีโอ
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Mycobacterium: Characteristics, Types, and Pathogenesis
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Actinomyces: Characteristics, Pathology and Treatment
มุมมอง 1.1K3 ปีที่แล้ว
#DRMAKKY #microbiology #microbiologystudent #microbiologylife #immunesystem #lifescienceskills Actinomyces: Characteristics, Pathology and Treatment. This video will teach you everything important about ACTINOMYCES. Actinomyces is a genus of the Actinobacteria class of bacteria. All are rod-shaped, non-spore former, G ve, facultatively anaerobic (except A. meyeri and A. israelii are obligate an...
Corynebacterium diphtheriae: Lab Diagnosis, Treatment, and Prophylaxis
มุมมอง 7423 ปีที่แล้ว
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Corynebacterium diphtheriae: Morphology, Diphtheria Toxin and Pathogenesis
มุมมอง 1.8K3 ปีที่แล้ว
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Antibody Structure, Types and Function
มุมมอง 2553 ปีที่แล้ว
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Characteristics and Types of Antigens
มุมมอง 5273 ปีที่แล้ว
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Immunizing Agents Classifications and Types
มุมมอง 8283 ปีที่แล้ว
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Endospore Stain Technique
มุมมอง 8K3 ปีที่แล้ว
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Gram Stain Procedure
มุมมอง 1.5K3 ปีที่แล้ว
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Bacterial Negative Stain Procedure
มุมมอง 5K3 ปีที่แล้ว
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Bacterial Simple Stain Procedure
มุมมอง 6K3 ปีที่แล้ว
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Bacterial Smear (Film) preparation practical steps
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#DRMAKKY #microbiology #labtechniques #lifescienceskills #microbiologystudent #microbiologylife In this video, we need to explain how to prepare the bacterial smear in the lab for bacterial staining preparation. th-cam.com/users/DRMAKKY drmakky101902.soci.vip/ #Facebook DR-MAKKY-102340904845636/?view_public_for=102340904845636 th-cam.com/video/opzHmjxPnbU/w-d-xo.html www.tubebuddy....
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جزاك الله خيرا🌸
If I treat cocs with plant base surfactant, then use the microbe to make sure the elimination of TPH on soil. Is that doable?
Can one take part in training in the lab.What is the tuition fee?
Thank you so much,l have learnt alot
Hello! May I know if do you have any technique to prevent the formation of condensate inside the tube or how do you remove the condensate inside the tube? Thank you!
can i get your mail id , for internship opportunity in your lab , i will be highly grateful. CURRENTLY I AM A STUDENT OF INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH .
hello i have question, if you put mineral oil in the slant for preservation, how do you get an inoculate from it if you need it?
This was so far an amazing video .
Thank you so much for this amazing information. lots of respect from Pakistan
please clean up that microcentrifuge ToT
I can tell where you are from, originally from the accent. Love the Egyptian accent! :). Great Video, thank y'all.
تحياتى ليك د عصام. محمد عبد البصير جامعة الازهر
I'm suffering from e coli problem like uti .when it stops 26 years old
Hello how are you now to this date, I'm suffering from it now I hope you are doing great pls I hope you can reply
السلام عليكم شكرا على التوضيح . اريد ان أسال عن أحسن مدرسة يمنية .،international لكي أسجل فيها ابنتي في السنة الثلاثة ثانويي رياضيات (مع العلم انها كانت أدرس بتونس )
Good job
Thanks
For any type of bacteria we can use this negative stain?
جزاكم الله خيرا على التوضيح
This principle same for all bacteria?
Thank you so much I got the the information that I need
اريد ان اقدم لابناىي في المدرسة الفلسطينية في ماليزيا كيف
السلام عليكم ورحمة الله وبركاته اخي كيف يمكن ان اتواصل معاك لاني ارا انك انسان يحب الخير للجميع
Thank you so much sir
Sir can you please provide subtitles in English?
موفق استاذ ممكن سؤال هل بكتريا diphtheria ممرضه تنمو عل وسط blood agar
Thanks for your question. Yup, this bacteria (C. diphtheria) can grow on Blood agar media with some morphological characteristics like gray or black colonies.
Very useful 👍🏻👍🏻👍🏻👍🏻💯
thanks for making video. if it is possible, can you tell me the media suitable for growing of actinomycetes.
Many thanks for your question. Actually, we have many media to culture the actinomyces, but the very common and easily to prepare it is starch-casein agar medium. You can search for full ingredients.
please can you do a video on PCR amplification method and 16s rRNA sequencing
Many thanks for your comment and I'll try my best to do so soon 👌👌
1.my question sir why freezing is done 2. and why freezing and heating is repeated twices
Sir how much U earn by taking Micro as a CAREER?
Prais GOD thanks doc
لو سمحت ضرورى لو مكتوب على زجاجة PC 7 للماء امن ولا غير امن ؟؟
I really appreciate your presentation doctor MAKKY.
13400'g' sir centrifuge is denoted by rpm , i little bit confusd
@Abhishek .. good question, please check this link for details tools.thermofisher.com/content/sfs/brochures/TR0040-Centrifuge-speed.pdf
Sir, i have a question! Can i use this method for rhizobial dna extraction?
Yes, you can
And harvesting Metals with plants 🪴 sounds like a win with me!!! I've been waiting to start this project with my lab!!!
I'm building my own genetics lab at home
Hi my name is Allen
Also sir, can you please post another picture of the DNA ring that you mentioned.
Sure I'll try to find a clear one .. Thanks
@@DRMAKKY sir, I tried this method but could not see any ring. Also my culture is from NB agar plate so is that creating it's difficult to see the ring? Since you are using McFarland.
@@DRMAKKY I am trying to isolate dna by other methods also but evey time I rna contamination. Is there any other way to remove rna contamination if we do not have rnase A
@@samridhipushkarna4162 I appreciate you asking. I believe you should try to increase the concentration of your bacterial suspension and/or double-check all of the reagents and extraction processes that were employed.
Hello sir Can we use the plates (inoculated and already kept in refrigerator for past 2-4 days) for isolation by this method? Or should the plates also be inoculated again for fresh batch? Second, if we have -80, for how long should we do that freezing step?
Thanks for ur good questions: #1 this depends on the growth rate of ur bacteria (slow growth rate u can use it for the past 2-4 days), if fast growth Ex. within 12-18hra better to get fresh culture); #2 in such case u can try for only 30 min OR 1hr for best results.
ممتاز يادكتور ربنا يعزك ويحفظك
thank you, sir I am thinking of doing my MSc thesis on this topic got a clear idea about biosurfactants in this video
Many thanks and Good Luck ..👌👌
المدارس الانترناشونال اختلاط ؟
نعم مختلظة بنين وبنات معا
The 260/230 value is very low.
Thanks a lot
Most welcome
جزاك الله الف خييير في ميزان حسناتك
معلم لغة عربية ازهري مصري لي مؤلفات في النحو والصرف مراجع منشورة بالهيئة العامة للكتاب بمصر اريد العمل بماليزيا
انا اعرف مدرس لغة عربية بيعمل فى ماليزيا .. انا ممكن ابعت لك رقمه وتتواصل معاه .. بس محتاج استاذنه اولا .. تحياتى
ي دكتور ممكن تساعدني كيف المدرسه اليمينه او المدرسه السعوديه هل مستواهم قوي في الانجليزي للثانويه و ارجوا الرد💐
شكرا على سؤالك .. فى الحقيقة هذه المدارس بتقوم بتدريس المنهج الخاص بالبلد نفسها وكأن الطالب بيدرس فى اليمن او السعودية يعنى باللغة العربية فبالتالى للأسف لا أعتقد مستوى الطالب فى الانجليزيه حيكون جيد
@@DRMAKKY طيب انا ميزانيتي ١٢٠٠ دولار هل ممكن اللاقي مدرسه حلوه بالإنجليزيبسعر هذا ؟
نرجو منك العوده لنشر فيديوهات الجميله والخاصه بالبكتريا 😍 يا دوك
ربنا يبارك فيكم .. قريبا ان شاء الله أكمل الفيديوهات .. مشغول كتير الفترة دى ومفيش وقت .. شكرا لكم وتحياتى
If lysozyme is not being added in case of gram positive bacteria