Abstract
Enzyme-Linked Immunosorbent Assay (ELISA), a core analytical method in biomedical research, has emerged as an indispensable tool for early disease diagnosis, drug discovery and screening, food safety monitoring, and more. Leveraging its exceptional sensitivity, high specificity, and relatively straightforward operational procedures, ELISA seamlessly integrates the highly specific recognition mechanism of antigen-antibody reactions with the potent catalytic capabilities of enzymes, enabling precise and quantitative detection of biomolecular targets.
Although the fundamental principles of ELISA are clear and operational procedures are trending towards standardization, practical applications still face challenges such as high background signals, unexpected results and insufficient color development. These issues directly compromise the accuracy and repeatability of test results. Therefore, ongoing optimization of experimental conditions and fine-tuning of operational details are crucial.
This WEBINAR will provide a comprehensive introduction to the core principles and standard operational procedures of ELISA technology. We will focus on addressing common challenges through scientific and effective solutions, and further to problem diagnosis and resolution. CUSABIO aims to enhance participants' understanding and use of ELISA technique, fostering its broader adoption in biomedical research.