วีดีโอ

can you please make videos on how to make primer sequence for genes , for performing rtpcr

Thank you very much, amazing presentation, very helpful. I am going to do tumor xenograft of acute myeloid leukemia. T

This video is so helpfull. Thank you so so much

Why did you choose to test only when you don’t know whether it is normal or not normal before choosing the test

thank you sir

Your explanation is mostly correct, expect for the OD being related to the protein expression. In that example you explained is a way to measure the amount of bacteria in the medium. We only add the IPTG when the OD is high (=lots of bacteria are there, around OD=7) and in your examplary images they will keep multiplying during the protein expression to an OD of 1. Also, your images are really small and the stuff you wrote in the red letter (6:00, f.e.) is not easy not read.

Thanks so muchhhhhh

Thank you so much sir 🙏

Thank you. Highly appreciable that you took your time and responded to our queries. It was really helpful. 😊🌸

Your explanations are best

Hello, sir. Can you please explain how to quantify in an excel file and graph plotting. Also, how to normalize it with loading control.

Sir very useful video thanks .

When does this methylation of the parent DNA occur? How did the parent DNA get methylated?

"Promo sm" ☀️

Excellent. Helpful video.

Helpful video thank you!

When I try to display a 2D diagram of the ligand in Discovery Studio, I receive an error message saying 'Ligand is not a single fragment' and I don't know what I need to do. I need help to resolve this issue.

Hii sir Thank u so much for sharing. Usually i scare ti study biochemistry.the way ur explained is simply superb. Pls do a video on contracon heracell 150i.it vil helpful for me. U know if anything i have to do independently I vil take decisions,theory class first.then i vil implement. Once again heartful thanks to u. For good video

How to make graph after getting mean of the all groups. ?

I have a question. In the case of antigen in the sample where you first added the antibody and it dried up, How do you achieve a control for that. Because since the antibody is loaded on to the membrane first. Won’t it bind the secondary antibody regardless of the binding to antigen. Also what’s the ratio of background when done ??

I’ve always had this question but no one seemed to know the answer, I now know why the DPN1 restriction enzyme cleaves only the parent strand but not the amplified strand. Truly one of the questions of all time.

THANK YOU SIR FOR UNDERSTANDING THIS

You have wechat. Are you based in China?

it says mobile selection must derive from one object only. what should ı do

Thanks for the effort but when you got to the actual overlap PCR part, you didn’t really explain clearly how it should be done.

can you please make a video about SOE PCR alone and explain it in very detail? It would be so helpful!

Thank you. Short, comprehensive, and complete.

SV40 causes cancer and is added to vaccines

Which protein you are quantifying?

Can you show primer designing for virus induced gene silencing experiments

Nice 👍

35 to 45 millimeter of mercury .I T IS PRESSURE UNIT

thank you very much for this video

In mixing step how two fragments will join as they are double stranded

can you please send the reference article for the formula of wound recovery %?

can we analyze DAB intensity with this method ?

Thank you so much.

Thank you for the sharing, normally how long will the processing take in genscript for the codon optimization to be completed? Thank you sir.

Thank you for this video!

They're disguising their eugenics. They're already aborting more black people than white, they already disallow disabled people to be born, more females are aborted than males, all advertisements of designer babies are images of white males. There's advertisements of anti aging which has nothing to do with sicknesses or diseases, if it was only about sicknesses and diseases they wouldn't of ever thought of touching the human germline. This is racism, sexism, ableism, communism, eugenics and capitalism. They do not have the right to mess with a person's genes just because they perceive something about them as a "problem". Autism is not a problem, they claim they value all humans equally, since they want to rub us out of existence they clearly do not see us as equal, they are full of discriminatory thoughts like Hitler doing eugenics. The problem is clearly they're view of autism and other primarily genetic conditions. I love being autistic, I wouldn't give it up for the world. Molecular biologist Miroslav Radman writes, "Mutagenesis has traditionally been viewed as an unavoidable consequence of imperfections in the process of DNA replication and repair. But if diversity is essential to survival, and if mutagenesis is required to generate such diversity, perhaps mutagenesis has been positively selected for throughout evolution." This will bring us to extinction. Especially since there's contradictory evidence. Evelyn Fox Keller explains: "We now know that mechanisms for enduring genetic stability are a product of evolution. Yet a surprising number of mutations in which at least some of these mechanisms are disabled have been found in bacteria living under natural conditions. Why do these mutants persist? Is it possible that they provide some selective advantage to the population as a whole? Might the persistence of some mutator genes in a population enhance the adaptability of that population? Apparently so. New mathematical models of bacterial populations in variable environments confirm that, under such conditions, selection favors the fixation of some mutator alleles and furthermore, that their presence accelerates the pace of evolution." The mutants behind autism and other conditions like Down Syndrome offer some great advantages to the human race, diminishing the genes is a great risk because without those mechanisms there is no asurety of genetic stability pushing us in the direction of extinction. Psychologist Howard Gardner warns: "With the coming of age of genetics, the danger magnifies. Beyond doubt we will discover genes that are important for reading alphabetical scripts; and there is already evidence that a small set of genes may be related to reading problems. As with the brain evidence, such information can be helpful for early intervention; but it could easily be used for stigmatising purposes. Indeed, it might become relevant for marriage prospects, holding a job, securing insurance, or even eugenic purposes. And no doubt, especially in our interventionist society, individuals with a genetic predisposition for reading problems will look into different kinds of genetic engineering or therapy. It is possible that such interventions will work and have no negative side effects, but it is perhaps more likely that they will have unanticipated effects. And we might even want to consider which valued human abilities - eg. spatial or pattern recognition skills - might be placed at risk were we to target our interventions specifically at reading disorders." They really want to destroy all alternative perceptions and ways of thinking. They're ignoring how many abilities they are going to destroy and how impoverished they are going to make our world because of their cultural myopia. Each time they have tried playing God they have only caused harm. Who caused the climate change? Scientists playing God trying to control nature, did the Gods anticipate the climate change? They are not only messing with humans, this whole earth is interconnected, they are messing with the entire ecosystem, with all life. How many species have been brought to extinction because of humans manipulating nature, there's endangered species today thanks to humans manipulating nature. If we fail to understand and take care of the natural world, it can cause a breakdown of these systems and come back to haunt us in ways we know little about. A critical example is a developing model of infectious disease that shows that most epidemics - AIDS, Ebola, West Nile, SARS, Lyme disease and hundreds more that have occurred over the last several decades - don’t just happen. They are a result of things people do to nature. The diseases they claim they want to cure were caused by doing this, so why are they doing it again? Was the world ready for COVID-19 to strike? I doubt it. World War II was caused by eugenics, why are they following Adolf Hitler's steps? Mutations are not random or accidental, malaria is endemic in Africa and Africans have developed mutations that protect them from malaria through adaptation, the sickle cell mutation is a defence mechanism against malaria. Europeans don't have these mutations, if a European goes to Africa they are more likely to get a disease. It was mutations that enabled the Europeans to survive the 14th century bubonic plague. Editing one gene may cure a disease but at the same time make them more susceptible to other diseases. Eliminate the sickle cell mutation from the gene pool and you've destroyed the only defence mechanism against malaria. Such foolishness. This is wicked and pure evil to think we don't deserve to be born just because we are different. CRISPR-Cas9 is a direct violation of human rights, especially human autonomy. They need to sort their discriminatory thoughts out and not touch us without our consent! "The Human Genome Project is founded upon a fallacy. There is no such thing as "the human genome." Neither in space nor in time can such a definite object be defined. At hundreds of different loci, scattered throughout the twenty-three chromosomes, there are genes that differ person to person. No body can say blood group A is "normal" and O, B, and AB are "abnormal." So when the Human Genome Project publishes the sequence of the typical human being, what will it publish for the ABO gene on chromosome 9? The project's declared aim is to publish the average or "consensus" sequence of 200 different people. But this would miss the point in the case of the ABO gene, because it is a crucial part of its function that it should not be the same in everybody. Variation is an inherent and integral part of the human - or indeed any - genome." The BBC Reported: This complete, single human genome will be a monumental technical achievement. Only 70 years have passed since the double-helix structure of DNA was first revealed, thanks in part to a grainy black and white image taken by Rosalind Franklin, transforming our understanding of how genetic information is stored. Today we have the capability to read the entire genetic 'textbook' that makes a person unique. But the geneticists involved say it is also a beginning, not an end. They now want to sequence the genomes of people from around the world, to build up a true picture of our species' genetic diversity. They want to understand what the previously unsequenced sections of DNA are doing. And they want to roll out end-to-end genome sequencing in clinics, to help doctors diagnose and treat us when we get sick. In short, the human genome will never be complete. We will never be done reading it. www.bbc.com/future/article/20230210-the-man-whose-genome-you-can-read-end-to-end

can you please share your number

that is very informative! thank you

Thank you so much. So helpful. How should we know that the p value under the 0.05 is signifucant? what is 0.05? Apart from imagej, Is that Excel you used for analyzing?🙂

Very insightful

Very important topic... But i wandered why your views are so less... Bcz you explain very nicely. So impressive ✌️

If we use primers 1F and 2R, we can decide the annealing temperature on the basis of the tm of these two individual primers but how to decide the extension time for this pcr? How will the two fragments eventually join? I can understand that the fragments would contain complementary regions but still in the 2nd pcr how will they join?

How crispr work is helpful in live imaging of DNA

God bless you öz abim 😀

Hi, thank you for this video. I would like to know if you know any software that can be used to design a set of primers for a complete genome sequence? I've been looking for the software. Thank you.

Hi. I watch your videos for site directed mutagenesis but still i have questions.Will you help me to design site directed mutagenesis primers?