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The HLA Channel
Brazil
เข้าร่วมเมื่อ 24 พ.ย. 2020
HLA-UERJ 30 ANOS!
O laboratório HLA-UERJ completou 30 anos em Outubro de 2024 e reuniu antigos e atuais funcionários para celebrar esta importante marca. Este vídeo é uma homenagem ao laboratório que moldou minha carreira acadêmica e profissional e continua oferecendo um serviço de excelência para o transplante no estado do Rio de Janeiro.
มุมมอง: 106
วีดีโอ
HLA by Nanopore technology in Peru
มุมมอง 634 หลายเดือนก่อน
This is "My FAS Life" series, where I show a little bit of my activities as a Field Application Scientist. But mainly I share here the new places I discover, the food, the culture, and the amazing people I meet. Join me by visiting Lima, Peru, and let's discover!
How I use NANOPORE in my HLA Lab
มุมมอง 884 หลายเดือนก่อน
Rafael Cita (Lab coordinator) and Tonny Marcos (Lab supervisor) tell us how their laboratory uses the Oxford Nanopore Technology to perform HLA typing.
What is CELL-FREE DNA and its importance for post-transplant monitoring
มุมมอง 1366 หลายเดือนก่อน
Quantitative analysis of cell-free DNA is recognized as a powerful diagnostic tool to monitor organ health. This non-invasive technique is based on the quantification of donor-derived, cell-free DNA (dd-cfDNA) circulating in the blood of the transplant recipient. Levels of dd-cfDNA may vary with different dynamics for each type of organ transplanted, but elevated levels of donor-derived DNA aft...
THE EUROPEAN HLA COMPANIES
มุมมอง 17611 หลายเดือนก่อน
OMIXON - GENDX - JETA - BAG - INNOTRAIN... Do you know these European HLA Companies? I visited some of these HLA companies to see what their headquarters look like. Are you a curious person in knowing about the companies that make the kits we use at our laboratories? So, stay with me, and let's discover!
ABTO 2023 - THE BRAZILIAN ORGAN TRANSPLANT CONFERENCE
มุมมอง 105ปีที่แล้ว
Join me in exploring the biggest conference on Transplantation in South America from the point of view of the HLA eyes. Many international speakers, renowned researchers, and Lab directors discussed the past, present, and future of HLA and organ transplants in Brazil and Portugal.
SBTMO 2023: The Brazilian Conference for Bone Marrow Transplantation
มุมมอง 136ปีที่แล้ว
Annually, SBTMO joins transplanters, nurses, laboratories, blood centers, and patients to discuss the transplant scenario in Brazil and the world. Have you ever seen how a Brazilian Meeting is? Take a look at this video to learn a bit about this fascinating world.
HLA EPLETS IN TRANSPLANTATION
มุมมอง 379ปีที่แล้ว
Hello, Guys! Welcome to THE HLA CHANNEL! Check out this paper about HLA EPLETS to learn more about this important concept in the HLA field.
WHAT IS NECESSARY TO BECOME A BONE MARROW DONOR?
มุมมอง 59ปีที่แล้ว
Deutsche Knochenmarkspenderdatei - DKMS. It is one of the biggest "Bone Marrow Donor Registry" in the world. I am in front of DKMS, in Berlin, Germany, to talk about bone marrow donation. Do you know what is necessary to become a bone marrow donor? What kinds of diseases are treated or cured with this donation? What diseases or conditions are impeditive to donation? Join me to learn about Bone ...
GVHD: IMMUNOLOGY CONCEPTS FOR EXPERTS
มุมมอง 74ปีที่แล้ว
Both acute and chronic GVHD involve distinct pathological processes, highlighting the need for stage-specific treatment approaches. Whereas acute GVHD has strong inflammatory components Chronic GVHD displays more autoimmune and fibrotic features. Check out this video to learn the immunological aspects of GVHD.
GRAFT-VERSUS-HOST-DISEASE (GVHD): BASIC CONCEPTS
มุมมอง 224ปีที่แล้ว
GVHD occurs when the donor’s T cells (the graft) view the patient’s healthy cells (the host) as foreign and attack and damage them. In this video, we can learn the basic concepts of GVHD and understand why it happens. Treatments, risks, and tips for patients can be found in this video.
PORQUE TESTAR VARIOS KITS DE HLA POR NGS?
มุมมอง 106ปีที่แล้ว
O laboratório de HLA do Hospital de Amor em Barretos - SP - Brazil, tem grande experiência na tipificação HLA por sequenciamento de Sanger e NGS. Neste vídeo, o Dr. Rafael Cita, coordenador do laboratório, compartilha conosco sua longa jornada através do sequenciamento, e explica porque ele possui vários kits de HLA por NGS no laboratório.
WHAT IS HLA? And why it is important in transplants...
มุมมอง 1.9Kปีที่แล้ว
HLA is important not only for immune system defense but also in transplantation outcomes. HLA typing is an important tool to access the genetic compatibility between recipients and donors. Recently, a new technology approach has become available for HLA tests. Watch this video to find out more about HLA typing done by Oxford Nanopore Technology.
SOFTWARE HLA TWIN: ¿CÓMO ANALIZAR UNA MUESTRA?
มุมมอง 30ปีที่แล้ว
Conceptos básicos del software HLA TWIN de Omixon.
COVERAGE. NOISE. PHASE. ALLELE BALANCE: Everything you must know when learning HLA analysis by NGS
มุมมอง 102ปีที่แล้ว
Hello, guys! Welcome to THE HLA CHANNEL. We have a special guest in this Video. Vinicius Stelet is an expert in the analysis of HLA by NGS. He knows every software there is in the market, and he shares his experience on how to start using the software with us. Coverage - Noise - Phasing - Allelic Balance. Ask your vendor about these features on your software. Let's discover!
CHECK YOUR FRAGMENTS: The most important tip when doing HLA by NGS
มุมมอง 105ปีที่แล้ว
CHECK YOUR FRAGMENTS: The most important tip when doing HLA by NGS
NANOTYPE: HLA EM ALTA RESOLUÇÃO PELA TECNOLOGIA NANOPORE
มุมมอง 1052 ปีที่แล้ว
NANOTYPE: HLA EM ALTA RESOLUÇÃO PELA TECNOLOGIA NANOPORE
THE OXFORD NANOPORE TECHNOLOGY: Best Review Ever!
มุมมอง 1282 ปีที่แล้ว
THE OXFORD NANOPORE TECHNOLOGY: Best Review Ever!
HIGH-RESOLUTION HLA TYPING IN LESS THAN 5 HOURS
มุมมอง 8552 ปีที่แล้ว
HIGH-RESOLUTION HLA TYPING IN LESS THAN 5 HOURS
MY FAS LIFE: NANOTYPE AND THE TRIP TO A COUNTRY CITY IN BRAZIL
มุมมอง 672 ปีที่แล้ว
MY FAS LIFE: NANOTYPE AND THE TRIP TO A COUNTRY CITY IN BRAZIL
NANOPORE MINION - HOW TO PRIMING THE FLOW CELL
มุมมอง 9022 ปีที่แล้ว
NANOPORE MINION - HOW TO PRIMING THE FLOW CELL
Flowcell Nanopore MinION: Protocolo para sequenciamento
มุมมอง 2852 ปีที่แล้ว
Flowcell Nanopore MinION: Protocolo para sequenciamento
Como preparar a Flowcell do Nanopore MinION
มุมมอง 502 ปีที่แล้ว
Como preparar a Flowcell do Nanopore MinION
ALL THE NEW HLA ALLELES DESCRIBED IN BRAZIL
มุมมอง 1152 ปีที่แล้ว
ALL THE NEW HLA ALLELES DESCRIBED IN BRAZIL
HOW MUCH IS IT PAID FOR AN HLA TYPING IN BRAZIL?
มุมมอง 963 ปีที่แล้ว
HOW MUCH IS IT PAID FOR AN HLA TYPING IN BRAZIL?
Great work Gustavo! Congrats. Nbrt
When you are re-using the same flow cell for another run, are you suppose to wash the flow cell again before priming the flow cell? Due to storing with storage buffer?
Hi! Thank you for your message! If you are going to reuse the flow cell in another sequencing run immediately after its use, you should wash the flow cell with DIL + WMX, but it is not necessary to add the storage buffer (S). Then, proceed with the priming of the flow cell and load the library. But if you will store the flow cell, even if for some hours, I recommend adding the storage buffer. Well, those are my recommendations! Let me know if you have any questions.
@@thehlachannel4307 Thank you! This clears the doubt!
Can i Do a flow cell / pore check directly after adding the storage Buffer? Because i want to see how many pores are left before putting the flow cell in the fridge.
Yes. The protocol suggests to do pore check
@@haranishan1529 Hi! I am sorry for missing your message. Yes, you can perform a flow cell check after adding the storage buffer. Old versions can present a bug after its first use. So, if by any chance your flow cell check returns zero pores, you can solve it by starting a sequencing and letting it run for some minutes. So the software will return the right number of the pores in the flow cell.
Hi when I use storage buffer it normally takes a couple days to get an accurate pore check
@@keithharrison8859 This is a useful information! Thank you!
Hello Sir ,thanks your video is very helpful . If possible I was wondering to know how many kit will need for type 60 samples and could you please share me the current cost . Very kindly🙏
Hello, Hana! Thank you for your comment on the video. The kit in the video has a 24-sample configuration, so you would need 3 kits to perform 60 samples. A 96-sample kit configuration is to be launched soon. For prices, please get in touch with me on LinkedIn and I can forward you to a salesperson: linkedin.com/in/gustavo-silva-5b237561
Okay well understood many thanks🙏
Many thanks for sharing this video! Do you have an idea about the price of the kit and the gene dx kits? and if there are any more kits from other companies! Thanks!
Hey! Thank you for your comment. Prices vary in each region, so it is hard to tell about prices here. More kits with the Oxford Nanopore Technology are coming. Starting with GenDx. And I am sure other companies are planning something with that technology.
Thank you for making this video! It is the only one that helped me decode my HLA results from Be the match (now under NMDP). Your video is very clear and easy to understand! Your accent and English makes no difference in getting your content across :)
Hi, Sarah! What a kind message. I am glad the videos help you!
What does it mean if a HLA allele can not be typed at all, even using high resolution techniques? For example, a lab report states for HLA-B a result of “B*18:01, B*- “. Is this a case of LoH? Or a novel allele? Thank you.
What a shame I didn't see your message before. In my view, if this result contains a loss of heterozygosity, it should be stated in the report. I would consider this result as a homozygous.
Thanks for the reply!@@thehlachannel4307
Hey man! Nice video! It was really useful! Don't bother with your accent! Everything that you said it's perfectly understandable. Keep up the good work! :)
Hi, Marcio! Thank you very much for your comment! Much appreciated!
Obrigada pela tradução!!!
Obrigado pelo comentario, Julia! Fico feliz em ter ajudado de alguma maneira.
Good..
Muchas gracias por tan excelente Presentación 🤩 . Podrías indicarme si este software y los reactivos son compatibles con secuenciador Ion Gene Studio S5 de Ion Torrent? Thank you
¡Hola Olga! Muchas gracias por tu comentario. El software TWIN es compatible con archivos generados por Ion Torrent. Pero los reactivos Omixon solo son compatibles con el secuenciador Illumina.
Don’t be a Follower, be a Student!!! 🤩🤩🤩
Thank you for your comment, Olga!
Eu gostei muito de este video 🤓
¡Muchas gracias por tu comentario, Olga! Espero que los videos te ayuden a aprender más sobre HLA.
thank you for showing me ....Las Vegas
Thank you for your comment, Juliana!
Muito obrigado!!!!!!!!!
Que comentario gentil! Obrigado!
MUITO BOM!!!🥰
Obrigado por seu comentário, Maris!
Muito obrigada por traduzir!!
Obrigado por seu comentário, Samanda!
Awnnn! You rock!!! 🥰
Thank you for your comment, Maris!
Muy interesante 🙋🏽♀️ gracias por compartir esto en español! ☺️ 👏🏽 sigue así !
Well done Gustavo !!! 🙋🏽♀️ thank you for sharing this! 🤓good job !
Thank you for your excellent work and review of the literature on this topic. Great job on your English as well!
Thank you very much for your kind message, Joy!
Thanks, Gustavo :). So proud to have you as a follower of the podcast!
It's an honor receiving your comment, Dr. Hitchman. Thank you!
Excellent video! I’m not from biological area, but still understood pretty much of the context. Congratulations!
Hi, Pedro! Thank you very much for your comment!
Congratulations on comprehensive explanation and visual artwork!
Thank you very much, Norbert!
Thank you for your presentation dr! Should I get tested HLA type test before covid vaccine? Which HLA test would you like to suggest before covid vaccine in order to prevent complications? Please let me know. Help me Dr! Thank you.
Hi, Namfom! Thank you for your comment. In order to know your HLA type, you can become a bone marrow donor volunteer. But be sure you will really donate bone marrow if you be selected for that. Knowing your HLA in advance will not give an advantage to your treatment, as the great majority of the physicians don't have this knowledge. The best prevention is still vaccine... and using a mask. Please, let me know if you would like further information.
Excelente
Obrigado por seu comentário, Laura!
Esse cara é incrível!
Obrigado, Michelle!
Parabéns por mais esse trabalho!
Excelente!
Muito bom mesmo
Obrigado por seu comentário, Romildo!
Aulão! Parabéns pelo conteúdo!
Valeu, Romulo!
Meu orgulho!!!
Excelente conteúdo, Romulo!
Parabéns, Romulo! Excelente palestra! Eu voto no tema 4 para o próximo vídeo hehehe