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Sir please provide this topic material Chromatography- General principles and classifications of chromatographic separations. Thin layer chromatography: Classification, principle, experimental technique, sample application, development of plate, retardation factor. Gas liquid chromatography: Gas liquid chromatography - instrumentation (columns and detectors), retention time and retention volume. Chromatographic behaviour of solutes, column efficiency and resolution, column processes and band broadening, time of analysis and resolution, Van-Deemter equation. Paper Chromatography: Principles, Rf values, experimental procedures, choice of paper and solvent systems, developments of chromatogram - ascending, descending and radial. Two dimensional chromatography, applications. Column Chromatography: Principles, experimental procedures, Stationary and mobile Phases, Separation technique. Applications. High performance liquid chromatography: Theory and instrumentation- column performance, gradient elution, delivery system, sample introduction, separation columns, detectors and applications of HPLC.

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Question 3 option c & d increasing the bond order and magnetic behaviour.then how to find the answer

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1) c 2)a

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