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Thanks for sharing. Can you please make a video on how to get composite using z-stacks

Thank you! very useful.

I have pics for MCF 7 cells in vitro stained by Phalloidin 488, how I can analyze them please? 😢

why do we have to converrt the images from index colour image to gray scale image, i mean is it a general standard for publication??

Hi, I am working on phases of polymer blends using Atomic force microscopy. Please upload a video about how to filter the phase image so that we can easily differentiate phases of the blend..

Quick question: why do you have to convert the images to 8-bit, instead of working directly with the separated channels, which are already in red-green-blue?. I usually just merge the channels without need to covert the images to 8-bit before.

Sir will you help me for some image processing, related to trichome (hair like structure on leaf) counting ? I am trying but not getting proper results.

Hello sir,I want to use this software to measure the width of the cracks of the reinforced concrete beams, if possible to apply a grid (perpendicular to the crack) on the image this grid to determine the width and thank you in advance please idea me it's urgent.

why you have first change the cokor to gray scale?? n on final click it became automatically colored one?

Please do video of how to scale images using image j

Why is the merged image saved in tiff?

Very good tute

💚| complete this series plz

Super helpful! Thank you

the voice and tone sounds like the listening test in TOEFL

Could you please tell me how to use pixel values at selected points in the image as numeric overlay?

Great!

Hi , I have a query regarding how to process the confocal images of cancerous cells after staining them with DAPI and mitotracker cmx ros? I want to quantify fragmented , intermediate and elongated mitochondrial morphologies. Please reach me at prayees26@gmail.com. thanks

Can you please show how to quantify signal intensity inside and outside nucleus and how to analyse colocalisation of two protein

Really well explained. Thanks

I need to find fibers of a filament in the series of images. each image might have hundreds or thousands of these fibers and I have hundreds of images. What is the systematic approach that can find these fibers in the image?

How can I ask software to segment part of an image? Is software able to detect the edges of a cell image from the background?

Can i find any video about the calculation of Intensity and related things which are required to be kept in mind while quantifying intensity and comparing in different biological replicates? will love to see if there is any. i just found one such video here on youtube. which lacks most of things

Could you please upload how to process a large-sized video in imageJ

Good to know about this video; you have presented very clearly. Would love to see videos for understanding co-localization analyses at Image J. Thank you !