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OGT
เข้าร่วมเมื่อ 20 ก.ค. 2016
As a leading global provider of diagnostic genomic solutions, our goal is to enable researchers and clinical decision makers to reach the right decisions for each patient, every time.
The quality and innovation built into our products for NGS, arrays and FISH reflects our commitment to improving the future of genetic clinical care.
We offer partnership and collaboration to our customers at every stage of their work.
The quality and innovation built into our products for NGS, arrays and FISH reflects our commitment to improving the future of genetic clinical care.
We offer partnership and collaboration to our customers at every stage of their work.
SureSeq Myeloid Fusion NGS Panel
The RNA-based SureSeq™ Myeloid Fusion Panel:
- Developed in partnership with myeloid cancer experts to the latest WHO guidance: Combining expert-led panel design with hybridisation-based enrichment for unparalleled uniformity and depth of coverage
- RNA-based, partner gene agnostic panel: Detect 30 common myeloid fusions plus novel fusion partners in a single cost-efficient assay
- Full assay and software solution: Streamlined library prep and intuitive, complimentary data analysis software - all backed up by OGT’s expert support
- Developed in partnership with myeloid cancer experts to the latest WHO guidance: Combining expert-led panel design with hybridisation-based enrichment for unparalleled uniformity and depth of coverage
- RNA-based, partner gene agnostic panel: Detect 30 common myeloid fusions plus novel fusion partners in a single cost-efficient assay
- Full assay and software solution: Streamlined library prep and intuitive, complimentary data analysis software - all backed up by OGT’s expert support
มุมมอง: 81
วีดีโอ
Getting ready for IVDR
มุมมอง 38ปีที่แล้ว
At OGT, we committed early to the In Vitro Diagnostic Regulation (IVDR) and have been working diligently over the last few years to meet the conformity requirements in a timely and compliant manner. We’re delighted that this hard work has led to the successful IVDR certification of our first CytoCell® fluorescence in situ hybridisation (FISH) probes that play a critical role in the management o...
Customisable aCGH microarrays complement NGS for additional insights into genetic disorders
มุมมอง 29ปีที่แล้ว
Dr Tracey Lewis delve into the benefits of combining the study of copy number variations (CNVs) and single nucleotide variations (SNVs) for enhanced mutation detection across a range of genetic conditions. Disclaimer: This presentation is intended for educational purposes only and does not replace independent professional judgment. Statements of fact and opinions expressed are those of the pres...
Advancing genomic interpretation
มุมมอง 71ปีที่แล้ว
Madhuri Hegde expands on her work with OGT combining NGS, microarrays - including OGT’s CytoSure® Disease-Focused Research arrays - and an all-inclusive genomic data interpretation platform for comprehensive analysis of genetic disorders. Disclaimer: This presentation is intended for educational purposes only and does not replace independent professional judgment. Statements of fact and opinion...
Validation of the CytoSure Constitutional v3 microarrays in prenatal and postnatal analysis
มุมมอง 20ปีที่แล้ว
Kris Van Den Bogaert discusses the evaluation of the CytoSure® Constitutional v3 arrays in prenatal and postnatal research. Disclaimer: This presentation is intended for educational purposes only and does not replace independent professional judgment. Statements of fact and opinions expressed are those of the presenters individually and, unless expressly stated to the contrary, are not the opin...
Implementation of the CytoSure Constitutional v3 microarray in the UK NHS
มุมมอง 28ปีที่แล้ว
Dom McMullan discusses the implementation of the CytoSure® Constitutional v3 array in the NHS laboratory, and how it facilitated cost-effective, evidence-based analysis of large populations. Disclaimer: This presentation is intended for educational purposes only and does not replace independent professional judgment. Statements of fact and opinions expressed are those of the presenters individu...
The whole exome: Completing the genomic picture using exon-targeted aCGH
มุมมอง 31ปีที่แล้ว
Lora Bean provides valuable insights into the use of arrays and NGS in parallel for CNV detection. While CNV calls from NGS data are becoming increasingly popular, they must be confirmed due to the significant false positive rate associated with them. Disclaimer: This presentation is intended for educational purposes only and does not replace independent professional judgment. Statements of fac...
Beyond CNV leveraging SNP arrays in cancer PNG
มุมมอง 43ปีที่แล้ว
Dr. Stephen Moore will share his experience utilising OGT's CytoSure® chromosomal microarrays in various tumour types. Specifically, he will highlight the different applications of the single nucleotide polymorphism (SNP) track beyond oligonucleotides confirmation. Disclaimer: This presentation is intended for educational purposes only and does not replace independent professional judgment. Sta...
Analytical utility assessment of myeloid NGS gene panel testing; a single site experience
มุมมอง 166ปีที่แล้ว
Dr. McCready compares a hybridisation-based NGS panel versus single analyte and amplicon-based NGS assays for somatic mutation testing in myeloid malignancies, from her experience with OGT’s SureSeq myPanel™ Custom Myeloid - 49 gene plus panel. Disclaimer: This presentation is intended for educational purposes only and does not replace independent professional judgment. Statements of fact and o...
Transitioning CLL FISH analysis into the molecular biomarker era
มุมมอง 41ปีที่แล้ว
Dr. Tucker shares her experience of how OGT’s NGS panel, SureSeq™ CLL CNV, has streamlined her laboratory workflow and enabled the detection of various aberrations in key genes implicated in CLL progression, including SNVs, indels, and CNVs. This alleviates the burden of running multiple assays and delivers comprehensive results with a single NGS analysis. Disclaimer: This presentation is inten...
Evaluation of an NGS assay for the detection of CNV & SNV acting synergistically to affect phenotype
มุมมอง 41ปีที่แล้ว
Dr. McCready will examine the utility of a CytoSure® Constitutional NGS panel to identify relevant variants in families with reduced penetrance or uncertain CNVs. Findings from this pilot cohort were compared to data from chromosome microarray analysis to evaluate feasibility of the NGS platform for copy number detection and its utility in identifying SNVs acting synergistically with CNVs to af...
Evaluation of CLL: Transitioning from a SNP-array CGH platform to an NGS-based CNV + SNV approach
มุมมอง 47ปีที่แล้ว
Dr Fortin will talk about transitioning from microarray to the SureSeq™ CLL CNV NGS panel, and how the SureSeq CLL CNV NGS Panel delivers a comprehensive genomic profile for each CLL sample using a single workflow, including trisomy 12, 11q, 13q and 17p deletions. Disclaimer: This presentation is intended for educational purposes only and does not replace independent professional judgment. Stat...
Technical & practical considerations of utilising OGT’s SureSeq CLL + CNV NGS panel in CLL research
มุมมอง 40ปีที่แล้ว
Presented at GLGC 2022, hear how our customers Dr. Peter Sabatini and Dr. Graeme Quest streamlined their CLL research and utilised OGT’s SureSeq™ CLL CNV NGS panel to detect CNVs in five chromosomal regions as well as SNVs and indels in key genes implicated in CLL. Disclaimer: This presentation is intended for educational purposes only and does not replace independent professional judgment. Sta...
Comparative analysis of testing methods used for the detection of PTDs in the KMT2A/MLL gene
มุมมอง 129ปีที่แล้ว
In this AMP 2022 session, Dr. Capo-Chichi shares his experience evaluating KMT2A- PTDs detection utilising three technologies; the SureSeq™ NGS panel with KMT2A specific probes engineered by OGT, an MLPA assay, and Optical Genome Mapping (OGM). SureSeq Myeloid NGS panels coupled with OGT’s Interpret NGS software provide robust detection of KMT2A-PTDs. Disclaimer: This presentation is intended f...
Comparison of mutation profiles in MDS and AML: A single site experience
มุมมอง 63ปีที่แล้ว
In this AMP 2022 session, Dr. McCready examines mutation profiles in AML and MDS from a large Southern Ontario cohort utilising two NGS panels, including OGT’s SureSeq™ Myeloid NGS panel. Dr. McCready illustrates how the SureSeq Myeloid NGS portfolio provides her lab with robust detection of somatic mutations in high GC- content genes such as CEBPA and structural variants such as IDTs in the FL...
NGS Workflow - Post-capture PCR, sample pooling, and sequencing
มุมมอง 1.1Kปีที่แล้ว
NGS Workflow - Post-capture PCR, sample pooling, and sequencing
NGS Workflow - DNA fragmentation, end repair, and 3’ end A-tailing
มุมมอง 4.7Kปีที่แล้ว
NGS Workflow - DNA fragmentation, end repair, and 3’ end A-tailing
SureSeq myPanel NGS Custom Cancer Panels
มุมมอง 434ปีที่แล้ว
SureSeq myPanel NGS Custom Cancer Panels
Chromosomal aberrations detected by FISH (US version)
มุมมอง 1.4Kปีที่แล้ว
Chromosomal aberrations detected by FISH (US version)
Chromosomal aberrations detected by FISH
มุมมอง 1.6Kปีที่แล้ว
Chromosomal aberrations detected by FISH
Streamline your CLL CNV analysis and alleviate the burden of running multiple assays
มุมมอง 1182 ปีที่แล้ว
Streamline your CLL CNV analysis and alleviate the burden of running multiple assays
Painless transition from arrays to NGS in ID/DD analysis
มุมมอง 1562 ปีที่แล้ว
Painless transition from arrays to NGS in ID/DD analysis
CytoCell FISH 'n' Tips: Reagent storage - Tips to prevent light exposure damage
มุมมอง 2552 ปีที่แล้ว
CytoCell FISH 'n' Tips: Reagent storage - Tips to prevent light exposure damage
CytoCell FISH 'n' Tips: Probe application - Tips to prevent patchy hybridisation
มุมมอง 2302 ปีที่แล้ว
CytoCell FISH 'n' Tips: Probe application - Tips to prevent patchy hybridisation
CytoCell FISH 'n' Tips: Probe analysis - Tips for FISH filter maintenance
มุมมอง 1772 ปีที่แล้ว
CytoCell FISH 'n' Tips: Probe analysis - Tips for FISH filter maintenance
CytoCell FISH 'n' Tips: Post hybridisation washes: Tips for minimising stringency issues
มุมมอง 3672 ปีที่แล้ว
CytoCell FISH 'n' Tips: Post hybridisation washes: Tips for minimising stringency issues
Mam how to identify tissue is digested or not
Mam how to mark in tissue area?Are u using H&E slides
Hi friends, can you tell us that what is the histoclear? we don't have this solution, can we use xylene instead of histoclear?
Thanks for your question. Histoclear is a de-paraffinisation agent. We use histoclear as an alternative to xylene in our lab, but we have many customers that use xylene for the de-paraffinisation step without issue.
Saline Sodium Citrate (SCC) buffer is an acid salt buffer solution and is used as a buffering agent to control acidity.
Why we put it in éthanol ?
Hi, thank you for the comment. The slides are washed in an ethanol series to dehydrate the sample prior to applying the probe. Hope this helps🙂
what model of thermotron did you use?
1:00 add homogeneous AMPure XP beads 2:40 “freshly made” ethanol 4:45 pour out “nuclease-free” water 6:45 supernatant
Do you wash streptavidin bead before?
That is correct! The streptavidin beads are washed/prepared in the previous step. For a look at how the streptavidin beads are prepared, watch our video: www.ogt.com/resources/ngs-resources-support/ngs-tutorial-videos-webinars/ngs-library-preparation-protocol-preparation-of-magnetic-beads/ For other NGS resources, access: www.ogt.com/resources/ngs-resources-support/
It was very informative.. Thanks a bunch 🙂😊
prof, u deserve a nobel prize~!
Why is that one in higher temperature and one in room temperature washes?
Dear Yakshaa Yathavan, thank you so much for your comment. You can find the answer to your question alongside other information in our FISH post-hybridization wash FAQs page: www.ogt.com/resources/fish-resources-support/fish-tips-troubleshooting/fish-faqs/post-hybridisation-washes/ Many thanks, the OGT team
Very helpful