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Stilla Technologies
France
เข้าร่วมเมื่อ 21 เม.ย. 2017
Stilla Technologies is a Paris-based Biotechnology which provides a cutting-edge digital PCR platform : The Naica System. Using the microfluidic Sapphire Chip, we offer the fastest time-to-result on the market with a unique 3-color multiplexing capability. Here you can discover the Naica System features, tutorials showing how to use our softwares and exciting news about our product and company !
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Thompson Jose Martin Anthony Anderson Deborah
I appreciate
Excellent video!
👌amazing technology
Where can i find the references?
Very helpful and informative Thankyou
Very interesting
Very nice video, i would be grateful if you could give me a reference of the Poisson law to convert the data into copies/µL/ Thank you very much !
Great.
Nice video! Does digital PCR also need to consider the effect of non-specific amplification caused by primer?
Slow down please
Nice
Le premier 👍👍👍
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Great tech, awesome video!
I liked your work. I have been trying to research for a vid that informs the stuff in this video! 👩⚕️Your tip totally reminds me of the channel of Dr. Ethan. Ethan's demonstrations are for sure educational and I learned a lot for my school. He is the most knowledgable Doctor on TH-cam and he teaches health symptoms and diseases! I recommend you see his channel out and give Dr Ethan a subscribe here! ➡️ #FutureDoctorEthan
What a great explanation, perfect for understanding this specially in Covid times.
Thank you : )
Thanks for the great explanation!
really it's great 👌
Isn't this qPCR?
amazing! thank you!
very clear explanation！
Why do you use fluorescence markers for the wild type allel in conventional qPCR if you are only interested in the mutation allel?
In qPCR, as for the digital PCR, the wild type strain is often considered a reference used to compared data relative to mutations. The dPCR is a method which allows the absolute quantification of a template (DNA or RNA), but it is also used to produce comparative (or relative) results, one example could be the necessity to compare the fraction of mutated sequences out of the wild type ones in a certain sample. At the following link www.gene-pi.com/tutorial/introduction-rare-mutation-detection-2-3/ you can find an example of an assay of this kind.
Why don't we construct a primer binding with the SNP therefore only the Mutant DNA would get amplified and we can visualize it
Of course ! This could be also one strategy. Depending on what are your expectaction you can design the assay you prefer. The naica® system offers the possibility to have multiple channels which could allow at the same time, and in the same experiment, the detection of different targets.
So you separate the sample before testing .?
Yes, the PCR mix is loaded in our chips chips and then is partitioned in thousands of small droplets in which the template sequences (DNA or RNA) will be randomly distributed. For more information about our workflow, please check on our website here: www.stillatechnologies.com/naica-system-workflow/
very clever technology!
Very nice technology and very good explanation, bravo!
This explanation was great! Thank you so much!
great video! best explanation of digital PCR I've seen so far
Thank you! If you are interested in learning more about Digital PCR please visit www.gene-pi.com/