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OiVM - Optical Imaging & Vital Microscopy
United States
เข้าร่วมเมื่อ 27 ก.ค. 2020
Optical Imaging & Vital Microscopy (OiVM) Core Facility at the Baylor College of Medicine
We are a Light Microscopy Core Facility Specializing in Optical Sectioning Tools for Fluorescence Microscopy
The mission of the Optical Imaging & Vital Microscopy (OiVM) Core is to give our investigators the tools and technical expertise they need to obtain reliable, reproducible scientific data using state-of-the-art microscopy modalities. We have over 20 years of experience educating, training and assisting scientists with experiments using the latest cutting edge imaging methodologies.
Our core specializes in vital and intravital imaging of cellular processes within cells, intact tissue explants, developing embryos and functioning organs within the live animal.
We are a Light Microscopy Core Facility Specializing in Optical Sectioning Tools for Fluorescence Microscopy
The mission of the Optical Imaging & Vital Microscopy (OiVM) Core is to give our investigators the tools and technical expertise they need to obtain reliable, reproducible scientific data using state-of-the-art microscopy modalities. We have over 20 years of experience educating, training and assisting scientists with experiments using the latest cutting edge imaging methodologies.
Our core specializes in vital and intravital imaging of cellular processes within cells, intact tissue explants, developing embryos and functioning organs within the live animal.
Training: 3D Image Reconstruction with NRecon
A descriptive software guide for reconstructing three dimensional (3D) data sets from X-Ray back projection images scanned on the Bruker Skyscan 1272 microCT.
For training on how to collect images for reconstruction, please see our full training video for the Bruker Skyscan 1272 microCT scanner here: th-cam.com/video/v9HZNvlzLCw/w-d-xo.html
00:00:00 - Introduction
00:00:36 - Starting NRecon Software
00:01:39 - Layout of NRecon
00:02:40 - Loading Dataset into NRecon
00:07:35 - Optimize the Reconstruction Parameters
00:08:11 - Misalignment Compensation
00:10:19 - Smoothing
00:11:59 - Object Larger than the Field of View
00:12:39 - Ring Artifacts Reduction
00:13:41 - Beam-Hardening Correction
00:14:33 - Cross-Section Rotation
00:15:07 - Initialize the Reconstruction Process
For training on how to collect images for reconstruction, please see our full training video for the Bruker Skyscan 1272 microCT scanner here: th-cam.com/video/v9HZNvlzLCw/w-d-xo.html
00:00:00 - Introduction
00:00:36 - Starting NRecon Software
00:01:39 - Layout of NRecon
00:02:40 - Loading Dataset into NRecon
00:07:35 - Optimize the Reconstruction Parameters
00:08:11 - Misalignment Compensation
00:10:19 - Smoothing
00:11:59 - Object Larger than the Field of View
00:12:39 - Ring Artifacts Reduction
00:13:41 - Beam-Hardening Correction
00:14:33 - Cross-Section Rotation
00:15:07 - Initialize the Reconstruction Process
มุมมอง: 3 691
วีดีโอ
Training: Bruker Skyscan 1272 microCT
มุมมอง 5K3 ปีที่แล้ว
A complete user training for acquisition of shadow projection images for 3D XRay microCT reconstruction using a Bruker Skyscan 1272. For the reconstruction of back projection images to 3D slices - please see our NRecon training video here: th-cam.com/video/9k8et7L62Bs/w-d-xo.html 00:00:00 - Introduction 00:00:37 - Overview of the Skyscan 1272 00:01:22 - Power on the Skyscan 1272 00:02:50 - Agin...
Using Immersion Objectives
มุมมอง 4.6K3 ปีที่แล้ว
An instructional video on using immersion objectives. We discuss why it is necessary to use immersion optics as well as how to properly use them on inverted microscopes. 00:00:00 - Introduction 00:00:25 - Why Do We Need Immersion? 00:05:56 - How to Use Immersion Fluid 00:15:05 - Positioning The Stage 00:16:59 - Changing Specimens 00:18:10 - Cleaning Up
Training: Advanced Airyscan Processing
มุมมอง 3.9K4 ปีที่แล้ว
An advanced discussion of strategies for improving the processing of Airyscan imaging data. This supplementary training offers additional recommendations on the secondary processing stages for 2D and 3D data sets. Before viewing this video, please see our Airyscan & AiryscanFAST training video here: th-cam.com/video/dBmPAvI1_ZQ/w-d-xo.html 00:00 - Introduction 01:51 - Processing 3D Images 07:39...
Training: Airyscan & AiryscanFAST
มุมมอง 16K4 ปีที่แล้ว
A comprehensive training module for the Airyscan & AiryscanFAST detection technologies of the Carl Zeiss LSM 880 confocal microscope. This training discusses the basic theory of Airyscan and AiryscanFAST imaging and provides a detailed discussion of how to use each mode for your experiments. The concepts discussed here rely on knowledge of the LSM 880 confocal microscope - and we recommend that...
Training: Carl Zeiss LSM 880 Confocal Microscope
มุมมอง 38K4 ปีที่แล้ว
Please watch our Introduction to Confocal Microscopy lecture before taking this training: th-cam.com/video/vn6app6XuII/w-d-xo.html A complete user training for the Carl Zeiss LSM 880 confocal microscope. This training takes the user from powering the instrument on through collecting 3 color confocal images with 3D Z-Stacks. This comprehensive training video is designed for Baylor College of Med...
Introduction to Confocal Microscopy
มุมมอง 59K4 ปีที่แล้ว
An introduction lecture on the basics of confocal microscopy. In this video you will learn about how fluorescence works, how a confocal image is formed and discuss the advantages and disadvantages of using this technology. This video is a prerequisite for training on any of the OiVM core's confocal microscopes.