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To measure the Instrument Response Function (IRF) of the FluoTime 300 system, you typically need a scatter sample that does not emit fluorescence. A common choice is a dilute colloidal suspension of nanoparticles like Ludox (colloidal silica), which efficiently scatters light but does not fluoresce. For example, take a drop of commercially available LUDOX TMA colloidal silica, 34 wt. % suspension in H2O, CAS Number: 7631-86-9 and dilute it 100 times with pure water. For further questions you may also send us an email to info(at)picoquant.com

Thank you for your interest in our confocal microscope MicroTime 200. The system is modular and is configured according to the customer's needs. Please contact us via info[at]picoquant.com if possible already with your requirements and application.

Thank your for your question. Both scenarios can happen, it depends on the signal intensity falling to the detector. These nanowire detectors were originally developed for real single-photon counting. The second half of Xiaolong Hu's presentation deals with detectors for multi-photon counting. For further questions you may also send us an email to info(at)picoquant.com

@@PicoQuant Ok I will definitely do if I require. But my question is if a stream of photons is allowed to fall over the nanowires , are the nanowires absorbing only one photon out of the stream and if yes then how???? Please explain

Hi. To measure hydrophobicity with FLIM you need a dye with different quantum yield in water compared to hydrophobic media. You can google "NBD dye". Some infos you can also find in our application note on FLIM in Confocal Microscopy Applications: www.picoquant.com/dl_technotes/AppNote_FLIM_2012.pdf. Please feel free to contact our support team directly with any questions at support(at)picoquant.com

The beam after pinhole is collimated an then focussed on the detector. The reason is that the active area of the detector is typically small

Yes. In the SAMI Workspace editor you can integrate EasyTau scripts into your method.

Thank you for your interest in our FluoTime 300 fluorescence lifetime spectrometer. Yes, the FluoTime 300 is collecting the data of each sample. The data can then be analyzed with the instruments software or exported as ascii. For further questions you may also contact us by email to info(at)picoquant.com

For measuring IRF in ideal case you should use the same emmision settings (for example filterset or wavelenght, intensity) and the same excitation settings (for example wavelenght, laser power, laser repetition rate) as for your sample. For real measurements, depending on the set-up type (spectrometer/microscope) or components (TiSa laser, diode laser, SPADs, PMT), variations of this general rule could be possible. For more details please also read our article about IRF measurements in our TCSPC Wiki: www.tcspc.com/doku.php/howto:how_to_measure_the_instrument_response_function_irf Please feel free to contact our support team directly with any questions at support@picoquant.com

Thank you for your comment and your questions. I have forwarded your questions to our support team who will contact you shortly. Concerning IRF measurements we have an article online in our TCSPC Wiki which might already carry all answers to your questions: www.tcspc.com/doku.php/howto:how_to_measure_the_instrument_response_function_irf Please feel free to contact our support team directly with any questions at support@picoquant.com

Thank you for your video. Can you suggest a website to find out the IRF for transient absorption,?

Hello Marinara, Thanks! The confocal volume can be (and has to be) calibrated by using a dye with known diffusion coefficient, such as ATTO488. A step-by-step tutorial on doing so can be found on the TCSPC Wiki: www.tcspc.com/doku.php/howto:calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script Best regards

Thank you for your comment. We will consider it and maybe we will make a video of it. PicoQuant offers the high performance luminescence spectrometer FT300. We can show you some performance tests of the FT300, but we don't know the properties of other spectrometer.

thank your for your request. Please contact us via our support request: www.picoquant.com/contact/support Best Wishes, Torsten

Hi jintaphorn klinsuk. Thank you for your interest in our system. Do you mean whether the Nikon A1R has a spectral detector? If so, this depends on the specific configuration of the Nikon A1. For further questions you may also contact our support team: www.picoquant.com/contact/support