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Chromatography Online
เข้าร่วมเมื่อ 25 ม.ค. 2022
Chromatography Online is the portal for users of chromatographic and mass spectrometric techniques.
Introduction to Multi Angle Light Scattering (MALS)
SEC-MALS - the combination of size-exclusion chromatography with multi-angle light scattering is an absolute technique for determining molar mass and size in solution.
มุมมอง: 50
วีดีโอ
Exploring the Power of Eluent pH as a Selectivity Tool for HPLC Method Development
มุมมอง 2514 วันที่ผ่านมา
In this seminar we discuss why selectivity should always be considered first in new method development activities or sample screening from both theoretical and practical viewpoints. For charged species, exploring mobile phase pH can be a powerful tool to influence selectivity and retention for: complex sample method development, peak shape optimisation, isolation work, or to ensure all analytes...
LC-MS for RNA Therapeutics to Ensure Product Quality and Process Consistency
มุมมอง 3421 วันที่ผ่านมา
The development momentum for RNA modalities has accelerated with the recent approvals of mRNA-based vaccines and RNA(CRISPR)-based gene therapies. These approvals have highlighted the need for the development of robust analytical methods for monitoring the relevant critical quality attributes (CQAs) of RNA molecules to ensure proper control of manufacturing processes and product quality. Oligon...
HPLC Troubleshooting, Column Care and Understanding Where Backpressure Comes From
มุมมอง 34หลายเดือนก่อน
Although HPLC method development has been improved by advances in column technology and instrumentation, problems still arise. Every troubleshooting guide mentions the reproducibility of selectivity as the most important column criterion; hence, most manufacturers have done their best to validate their production processes. Nevertheless, small differences that exist in surface chemistry tend to...
Understanding Reverse Phase Selectivity for Different Compound Classes
มุมมอง 41หลายเดือนก่อน
Are you new to HPLC method development, or maybe you would welcome a quick refresh? This webinar will show you how to select an appropriate column based on the types of molecules you are looking to separate, and then highlight how choice of mobile phase can be optimized to help maximize selectivity. The focus of this webinar will be the reversed phase separation of small molecules, considering ...
ESI vs APCI. Which ionization should I choose for my application?
มุมมอง 212หลายเดือนก่อน
Many analysts never consider anything beyond the electrospray interface for LC/MS. Electrospray is undeniably a very flexible technique. It works well for macromolecules as well as small molecules, positive and negative ion mode, and is a great choice for thermally labile compounds. However, it is not the optimum interface for all organic molecules and, in fact, does not work at all for some cl...
Streamlined Reversed Phase Method Development Using a Column Screening and Software Modelling
มุมมอง 33หลายเดือนก่อน
The adoption of a structured approach to method development has the potential to provide significant savings in both development time and costs. Column screening is a widely adopted strategy that explores selectivity - the key to analyte resolution. The most promising result from screening experiments may then be taken for further optimisation to produce the final analytical method. The use of ...
Oligonucleotide Analysis. Practical Implementation Approaches for HPLC and LC-MS Analyses
มุมมอง 732 หลายเดือนก่อน
Oligonucleotides represent a diverse class of therapeutics that provide great promise to treat disease, but can pose a number of analytical challenges to scientists. In this webinar, we will present ion exchange and reversed-phase-ion-pair approaches to the separation of nucleic acids, separation examples of from small modified RNA, phosphothioated oligonucleotides, plasmid uncoiled and superco...
GC Columns Care & Troubleshooting
มุมมอง 182 หลายเดือนก่อน
The objective of this webinar is to guide GC users on GC column care & troubleshooting. Audience will learn about the ways to maintain GC column performance. Guidance to troubleshoot common issues in GC separation will be share.
Measuring Peak Separation Quality
มุมมอง 282 หลายเดือนก่อน
From this video you will discover the methodologies for calculating resolution, asymmetry, selectivity, efficiency and learn how to quantify the quality of a separation, along with a reference of what each value could and should be.
How to Optimize Biochromatography Separations
มุมมอง 312 หลายเดือนก่อน
Ion exchange chromatography is useful in the analysis of enzymatic post-translational modifications and chemical modifications that can occur on a bio-therapeutic. Understanding column selection and buffer optimization (salt vs pH gradients) is key to developing a robust, high resolution, and sensitive assay.
ASTM D7823 Method for Analyzing Regulated Phthalates in a Complex Matrix
มุมมอง 1413 หลายเดือนก่อน
This webinar is the basis of the existing ASTM D7823 method for regulating phthalates using the micro-furnace TD-GC/MS technique. In this webinar, you will learn how to identify and quantify the phthalates in a complex matrix and replace the traditional solvent extraction techniques with thermal extraction for the most accurate and automated results.
Introducing HILIC Theory and Practice Including a Step by Step Guide to HILIC Method Development
มุมมอง 513 หลายเดือนก่อน
In this Webinar we will present the range highlighted below and discuss how these columns can offer improved method development strategies in HILIC conditions.
The Differences Within C18 Columns
มุมมอง 353 หลายเดือนก่อน
When it comes to LC analysis, a common question for users is, “Which LC column should I choose?”. The column is the heart of the LC system, which plays a pivotal role in ensuring high-resolution separation. The fundamental knowledge of column selection can hence help you decide the optimal column for your specific application. In this webinar, we will introduce the different parameters involved...
Mobile Phase Selection in HPLC & UHPLC
มุมมอง 1073 หลายเดือนก่อน
A number of topics will be covered during the seminar: solvent options for HPLC, method development solvent considerations, isocratic and gradient chromatography, influence of buffer salt and pH on separations.
Hydrogen for Pesticide Residue Analysis
มุมมอง 624 หลายเดือนก่อน
Hydrogen for Pesticide Residue Analysis
Fast, Accurate, and Cost-Effective AAV Characterization with LC-MALS
มุมมอง 234 หลายเดือนก่อน
Fast, Accurate, and Cost-Effective AAV Characterization with LC-MALS
A Comprehensive Food Safety Analysis from Initial DIA Screening to Automatic Reinjection
มุมมอง 334 หลายเดือนก่อน
A Comprehensive Food Safety Analysis from Initial DIA Screening to Automatic Reinjection
Method Development for Complex Samples
มุมมอง 674 หลายเดือนก่อน
Method Development for Complex Samples
10 Most Important Maintenance Points Your Need to Know about Your Split/Splitless Injection System
มุมมอง 505 หลายเดือนก่อน
10 Most Important Maintenance Points Your Need to Know about Your Split/Splitless Injection System
Improving GC×GC Workflows for Petrochemical Analyses
มุมมอง 575 หลายเดือนก่อน
Improving GC×GC Workflows for Petrochemical Analyses
Understanding How Good Laboratory Practice Minimizes Instrument Downtime
มุมมอง 465 หลายเดือนก่อน
Understanding How Good Laboratory Practice Minimizes Instrument Downtime
How to Implement Large Volume Injections with the MMI Inlet
มุมมอง 1595 หลายเดือนก่อน
How to Implement Large Volume Injections with the MMI Inlet
Solid Phase Microextraction SPME. How Does it Work for My Samples
มุมมอง 3365 หลายเดือนก่อน
Solid Phase Microextraction SPME. How Does it Work for My Samples
High-Sensitivity Quantitation of Nitrosamine Impurities in Drug Substances Using LC-MS/MS
มุมมอง 2876 หลายเดือนก่อน
High-Sensitivity Quantitation of Nitrosamine Impurities in Drug Substances Using LC-MS/MS
Hydrophobic Interaction Chromatography as a Platform for Characterizing Antibody Drug Conjugates
มุมมอง 1286 หลายเดือนก่อน
Hydrophobic Interaction Chromatography as a Platform for Characterizing Antibody Drug Conjugates
Covering your Streams with Gas Chromatography
มุมมอง 486 หลายเดือนก่อน
Covering your Streams with Gas Chromatography
Ok thanks for the presentation.. but just gotta say, what dafuq up with the sound dropping in and out? greatly impinges on the watchability of the video. Something to be aware of when recording new videos in the future. Kind regards.
its nothing but only slide reading
Highly informative.
Thank you for the video. It is really clear and helpful. But in our lab, it is very strange that our GC-SCD gives decaying signals time by time. We tried to inject Sulfur standard gas and each time the peak area reduced by 2~3%. But if we let the instrument take a break for one hour, the signal rises in the next shot. Agilent Engineer came and replace the loop, valve and something else, but it has no change. We are still trying to solve the problem.
Voice quality is poor. Please try to address this for future presentations.
So helpful for my exam, thank you!!!
very informative
Very helpful thankyou
Very interesting , thanks for sharing
Good one
thank you so much for sharing these webinars :)
Thank you so much
Nice presentation, high level demonstration of scientific principles on ms ms analytical technology.
32:48
Thanks. Can we able to predict the no of isomers present in the mixtures using the LCMS/MS
It depends. Some isomers will react differently with HPLC column, and therefore will have different retention times. For example, a branched chain isomer will typically have less interaction with the packing of the column, and will elute early, while a straight chain isomer will tend to have more interaction with the column and will elute later. In cases like this, you will see multiple peaks in a chromatogram, which each represent a different isomer. However, this is very dependent on the type of isomer (for example stereoisomers, or large molecules with isomers that are very similar in structure will be difficult to separate) and also dependent on the relationship between the compound and the column, and the mechanism of interaction between the two. I hope this is helpful.
@@Ccccccccccsssssssssss Thankk you sir
Impressive … perfectly covered all the points.. Thanks for the detailed explanation
Good
Nicely presented😀
Not this brand user but its general so its important
very nice , thanks a lot for such a guidance
thanks sir , i got a crystal clear clarity on subject , very nice explanation , thanks a lot for beautiful guidance .
Cleaning source 43:00 great video. thanks!
Thanks!!!!!
I currently work for ThermoFisher scientific in the UK but I am studying my Masters degree in environmental assessment and control. This video was helpful in my paper on methods of testing drinking water for fluoride contamination.