This video was recorded by the Live Cell Imaging facility at the Karolinska Institute in Sweden during the LCI course 2024. It is intended as teaching material for microscopy users and facilities. Feel free to use it but please, mention the Live Cell imaging facility at KI, Sweden as the source of the video. You can also become a follower of our microscopy blog where we share tips and tricks about light microscopy (microscopykarolinska.se) or visit our website (ki.se/en/bionut/welcome-to-the-lci-facility).