วีดีโอ

Greetings, professor Hiroshi T I am currently working on quantification of cellular lipids. I have a few questions on “notame” 1) Is notame application can be used for lipidomics..? 2) can we perform quantification using notame. If possible please make a video on interpretation of multivariate analysis data. Thanks in advance.

Thank you so much for your helpful podcasts.

The possible complication from supplementing Omega3's and allowing for increased motility in-and-out of the cells of CD4 and other immunological variants could be "immuno-senescence". Has there been long term studies looking into this question?

Thank you Maria for this introduction!

Pls send me this ppt🙏🙏🙏🙏

What should we select the reference from setting Analysis parameter setting? is this the blank file. And what is the reference file for isotope tracking? is this the same blank file?

I dont understand the enrichment scheme though, why is red enrichhed when it represents only 4 out of 21 elements, then why is square enriched when there are only 6 squares compared to 7 circles. The video does not explain why they used one tailed Welch test to rank the lipids and what they compared in the Welch test. If they compared lipid by lipid in ER vs. PM, then their lipids might be down- or up-regulated so a two-tailed test would be more appropriate....etc

I have 45 file done by GCMS and when i click on PLS , software needs me to put the Y variables , what are the Y variables? I have another question if i have an internal standard file , what is the type of that file ( options are sample, blank, QC , Standard) no internal standard option what can i do? , 3rd question regarding the alignment excel sheet exported ( i do not have the area of most of the compounds, i have -2, 0, -1 instead ) what is the problem ?thank you

Wonderful video and solved my curiosity regarding lipidomic dataset outlook. Thanks

Video is quite new, but does not contain crucial information from patient's standpoint of vview. For instance, cirrhosis is reversible in about 30% of pations on ketogenic diet. We've known this from stusies for some time now. Mice are not good model of human metabolism in general even though they;re convinient animal to study and manipulation. This whole model is fixed on drugs use. But every known drug has small healing effect in comparition to low carbohydrate diet. When carbohydrates are low, high fat intake does not lead to NAFLD in humans. It even reverses majority of stages of this disease provided that fats are not eaten in form oils extracted from seeds or industrially produced margarines. Having said it, drugs have their place in treatment, but in most cases (excluding cancer) they are secondary and adjunctive and not being main healing factor. The main strategy is restriction of fructose (eaten or produced in the body), high amounts of carbohydrates and harmful fats.

lumineux et gratifiant

Is there any difference between oxysterols chemical structure with oxide cholesterol which causes the atherosclerosis?

Great presentation! CNS

Also did you ever look at their effect on "Redox Stress"?

What causes "Yellow Fat Disease" in some animals like cats who eat too much Omega-3 from Tuna? Does this correspond to risk in humans?

Instead of studying the supplementing of Omega-3 why not study the restriction of Omega-6? The work of Bruce Hammock and Covid-19, mentioned in this presentation, points to the Leukotoxins as correlated with disease severity but what if FFA release was inhibited like with aspirin? Or does aspirin only target COX and not LOX? Does vitamin E have any inhibitory action on these Leukotoxins?

I want to do the analysis but don't know how to arrange data (which is in excel format )to upload it. Please help.

Hi! I dont do lipidomics. In any case I wanted to thank you for such an illustrative and useful video. I was a little bit lost with MZmine and I see a lot of posibilities now. Still need to figure out some other things with my data set but thanks a lot :)

How did he got the reference database for lipids (in identification section at 15:53 min) ? Is that available online or do we need to make it by ourself ? I am working with yeast lipids, what type of database would be appropriate for me ? Thank you.

Magnificent lesson

Thank you so much. this video is very helpful and so nicely explained. I just did not understand one thing why you multiply the concentration values with 2 during normalization.

Very informative! Thank you Dr Dennis

thx for your effort it is really helpful I hope I can find another video to explain more about the quantification of oxysterols using LC-MS\MS .

Why nobody commenting like bro its pure knowledge

Insane. Thank you very much for this thorough presentation!

I am sorry that can you record the voice again. It is not understandable.

If applicable, is there a platform that applies the same principle for other omics like metabolomics pathways?

Thanks a million for this knowledge, extremely valuable!

nice summary of lipidomics MS

Fascinating webinar! Thank you very much! The only thing that lacks for me is literature citations, so it would have been possible to read more deeply into the particular studies

Thank you for keeping these recordings online!

Huge thanks for this very interesting and informative webinar.

Loading data on this tool is a nightmare. Could you please help me to sort the correct format of my data as I tried all the possibilities after reading the documentation but nothing is working for me. I can only load the demonstration file and when I try to load my own data tool is continuously giving me errors.