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Dr. Carla Ramos
United States
เข้าร่วมเมื่อ 12 ต.ค. 2011
New Youtube channel with tutorial videos
Hello,
I am going to be closing this youtube channel however all the tutorial videos have been transferred to the new channel called Ciencia y Science. If I have not answered a question on this channel please go to the new channel, post your questions so I can answer them. Videos will remain here until November then they will be deleted.
Stay tune for new tutorial videos to come.
Here is the link to the new youtube channel
th-cam.com/channels/vOlUnIRNhI2PonooNtcJ7w.html
I am going to be closing this youtube channel however all the tutorial videos have been transferred to the new channel called Ciencia y Science. If I have not answered a question on this channel please go to the new channel, post your questions so I can answer them. Videos will remain here until November then they will be deleted.
Stay tune for new tutorial videos to come.
Here is the link to the new youtube channel
th-cam.com/channels/vOlUnIRNhI2PonooNtcJ7w.html
มุมมอง: 502
วีดีโอ
Testing the Neon Transfection System with 100uL tips
มุมมอง 1.1Kปีที่แล้ว
If you have questions or comments please go to the new channel Ciencia y Science and ask your questions there so that I can answer them. th-cam.com/channels/vOlUnIRNhI2PonooNtcJ7w.html In this short video I show how to use the Neon transfection system using the 100uL tips, E2 buffer and 1600V. For my final protocol using the Neon Transfection system with primary mouse retinal endothelial cells ...
Neon Transfection System using10uL tips
มุมมอง 1.1Kปีที่แล้ว
If you have questions or comments please go to the new channel Ciencia y Science and ask your questions there so that I can answer them. th-cam.com/channels/vOlUnIRNhI2PonooNtcJ7w.html In this video I'll show you how to use the Invitrogen Neon transfection system to gene silence mouse VCAM-1 in mouse retinal endothelial cells. Time Stamp 0:00 Introduction to the Neon Transfection System 5:28 My...
Measuring protein concentration and sample denaturation
มุมมอง 6K2 ปีที่แล้ว
If you have questions or comments please go to the new channel Ciencia y Science and ask your questions there so that I can answer them. th-cam.com/channels/vOlUnIRNhI2PonooNtcJ7w.html In this tutorial video I have included both 1) measuring protein concentration from sample lysates using the Pierce BCA protein assay and 2) Denaturing sample lysates using 4X Laemmli buffer Time stamp 0:00 Intro...
Denaturing protein samples for SDS-PAGE or western blotting
มุมมอง 2.1K2 ปีที่แล้ว
If you have questions or comments please go to the new channel Ciencia y Science and ask your questions there so that I can answer them. th-cam.com/channels/vOlUnIRNhI2PonooNtcJ7w.html In this tutorial video I am showing you how I prepare my lysate samples once I've completed the BCA assay to determine total protein concentration in my samples. I will go over my calculations and how I prepare t...
Pipetting Tutorial
มุมมอง 6972 ปีที่แล้ว
If you have questions or comments please go to the new channel Ciencia y Science and ask your questions there so that I can answer them. th-cam.com/channels/vOlUnIRNhI2PonooNtcJ7w.html In this tutorial video I'll show you how to use pipettors with appropriate tips. I'll also show you the correct way to pipette using the two stops. Hope this helps you in your experiments. If you have any questio...
Western Blot Part 1
มุมมอง 7K2 ปีที่แล้ว
If you have questions or comments please go to the new channel Ciencia y Science and ask your questions there so that I can answer them. th-cam.com/channels/vOlUnIRNhI2PonooNtcJ7w.html This tutorial video introduces one of my favorite lab techniques, western blotting. Western blot technique is used to isolate, visualize, and study proteins. This is the 1st part of a series of western blot techn...
cDNA synthesis Tutorial
มุมมอง 40K2 ปีที่แล้ว
If you have questions or comments please go to the new channel Ciencia y Science and ask your questions there so that I can answer them. th-cam.com/channels/vOlUnIRNhI2PonooNtcJ7w.html The cDNA synthesis tutorial video is the follow up step after isolating RNA. We are able to synthesize cDNA from RNA through a process called reverse transcription (RT). RT is a process that does not occur in mam...
Running qPCR of cDNA
มุมมอง 26K2 ปีที่แล้ว
If you have questions or comments please go to the new channel Ciencia y Science and ask your questions there so that I can answer them. th-cam.com/channels/vOlUnIRNhI2PonooNtcJ7w.html This tutorial video is a follow up of the RNA isolation video. Here I show the qPCR set up and process. I used mouse retinal micro-endothelial cells (MRMEC) cDNA and primers from TaqMan system. [Time stamps] 00:0...
Isolating RNA from primary cells
มุมมอง 9K2 ปีที่แล้ว
In this tutorial video I am isolating RNA from primary mice retinal cells using the Qiagen RNeasy Mini kit. Note that I kept saying 800 instead of 8000xg, it was a long day :-) The liquid Nitrogen tank decided to start screaming at time 22.25 - 22.27 minutes, please disregard him.