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Leica Microsystems Tutorials
Germany
เข้าร่วมเมื่อ 8 มิ.ย. 2020
Welcome to Leica Microsystems Tutorials!
Explore the world of microscopes and scientific instruments and get detailed applications tutorials from our team of Advanced Workflow Specialists.
Main channel: th-cam.com/users/leicamicrosystems
Explore the world of microscopes and scientific instruments and get detailed applications tutorials from our team of Advanced Workflow Specialists.
Main channel: th-cam.com/users/leicamicrosystems
Automated cell counting with the Mateo FL Digital Fluorescence Microscope
Obtain a swift and accurate cell count with Mateo FL in just 5 seconds, using automated cell counting, instead of 15 minutes of manual counting.
Choose Mateo FL for reliable, reproducible advanced cell checks: www.leica-microsystems.com/products/light-microscopes/p/mateo/
#digitalfluorescencemicroscope #confluencyanalysis #MateoFL
▬ Transcript ▬▬▬▬▬▬▬▬▬▬▬
Automated cell counting with the Mateo FL Digital Fluorescence Microscope
Obtain a swift and accurate cell count with just a few clicks on your Mateo FL and forget about tedious manual counting.
Place your sample on the stage.
Now select your desired channel. You can choose between “Phase Contrast” for the transmitted light option or one of the fluorescent channels.
Now use the focus knob to adjust the focus, as well as the object guide to select the desired field of view.
Once you are happy with your selection, simply click on “AI module” and go to “Cell Counting”.
Measure the diameter of your cells manually or automatically. And few seconds later you get
your cell count.
Mateo FL: for reliable, reproducible, advanced cell checks
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Discover our LinkedIn Medical Channel: www.linkedin.com/company/leica-microsystems-medical/
Find us on Facebook: LeicaMicrosystems
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Subscribe @LeicaMicrosystems @LeicaMicrosystemsTutorials
▬ About Leica Microsystems ▬▬▬▬▬▬▬▬▬▬▬▬
Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures. Ever since the company started as a family business in the nineteenth century, its instruments have been widely recognized for their optical precision and innovative technology. It is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopes. Leica Microsystems has six major plants and product development sites around the world. The company is represented in over 100 countries, has sales and service organizations in 20 countries, and an international network of distribution partners. Its headquarters are located in Wetzlar, Germany.
Copyright© 2024 Leica Microsystems MC-0007678
Choose Mateo FL for reliable, reproducible advanced cell checks: www.leica-microsystems.com/products/light-microscopes/p/mateo/
#digitalfluorescencemicroscope #confluencyanalysis #MateoFL
▬ Transcript ▬▬▬▬▬▬▬▬▬▬▬
Automated cell counting with the Mateo FL Digital Fluorescence Microscope
Obtain a swift and accurate cell count with just a few clicks on your Mateo FL and forget about tedious manual counting.
Place your sample on the stage.
Now select your desired channel. You can choose between “Phase Contrast” for the transmitted light option or one of the fluorescent channels.
Now use the focus knob to adjust the focus, as well as the object guide to select the desired field of view.
Once you are happy with your selection, simply click on “AI module” and go to “Cell Counting”.
Measure the diameter of your cells manually or automatically. And few seconds later you get
your cell count.
Mateo FL: for reliable, reproducible, advanced cell checks
▬ Follow us ▬▬▬▬▬▬▬▬▬▬▬▬
Discover us on LinkedIn: www.linkedin.com/company/leica-microsystems
Discover our LinkedIn Medical Channel: www.linkedin.com/company/leica-microsystems-medical/
Find us on Facebook: LeicaMicrosystems
Follow us on Twitter: LeicaMicro
Follow us on Instagram: leica.microsystems
▬ More Videos ▬▬▬▬▬▬▬▬▬▬▬▬
Subscribe @LeicaMicrosystems @LeicaMicrosystemsTutorials
▬ About Leica Microsystems ▬▬▬▬▬▬▬▬▬▬▬▬
Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures. Ever since the company started as a family business in the nineteenth century, its instruments have been widely recognized for their optical precision and innovative technology. It is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopes. Leica Microsystems has six major plants and product development sites around the world. The company is represented in over 100 countries, has sales and service organizations in 20 countries, and an international network of distribution partners. Its headquarters are located in Wetzlar, Germany.
Copyright© 2024 Leica Microsystems MC-0007678
มุมมอง: 290
วีดีโอ
Measure transfection efficiency with the Mateo FL Digital Fluorescence Microscope
มุมมอง 2176 หลายเดือนก่อน
Reliably and accurately check cell transfection efficiency with Mateo FL, eliminating the need for subjective estimations. Choose Mateo FL for reliable, reproducible advanced cell checks: www.leica-microsystems.com/products/light-microscopes/p/mateo/ #digitalfluorescencemicroscope #transfectionefficiency #MateoFL ▬ Transcript ▬▬▬▬▬▬▬▬▬▬▬ Measure transfection efficiency with the Mateo FL Digital...
Confluency measurement with the Mateo FL Digital Fluorescence Microscope
มุมมอง 2096 หลายเดือนก่อน
Find out how Mateo FL helps you eliminate guesswork and increase confidence in your downstream experiments using AI-assisted confluency measurements. Choose Mateo FL for reliable, reproducible advanced cell checks: www.leica-microsystems.com/products/light-microscopes/p/mateo/ #digitalfluorescencemicroscope #confluencyanalysis #MateoFL ▬ Transcript ▬▬▬▬▬▬▬▬▬▬▬ Confluency measurement with the Ma...
Unboxing and getting started with the Mateo FL Digital Fluorescence Microscope
มุมมอง 3596 หลายเดือนก่อน
Watch the video to find out how to easily set up your Mateo FL digital fluorescence microscope. Choose Mateo FL for reliable, reproducible advanced cell checks: www.leica-microsystems.com/products/light-microscopes/p/mateo/ #digitalfluorescencemicroscope #MateoFL ▬ Transcript ▬▬▬▬▬▬▬▬▬▬▬ Unboxing and getting started with the Mateo FL Digital Fluorescence Microscope Welcome to Leica Microsystems...
UC Enuity Ultramicrotome - Automatic block face trimming in 2D
มุมมอง 46710 หลายเดือนก่อน
Empower your team through automation! Trimming a blockface is a time-intensive task and takes a certain level of training. UC Enuity takes over this task and handles the trimming. Simply define the boundaries of your sample block and walk away. 00:00 - Unveiling the UC Enuity Ultramicrotome 00:25 - Loading sample and knife 01:04 - Automated sample and knife movement 01:27 - Trimming in process ...
UC Enuity Ultramicrotome - Trim your target with the help of fluorescence
มุมมอง 33710 หลายเดือนก่อน
Trimming a block face is a time-intensive task and takes a certain level of training. In addition, sometimes the sample is buried inside of the resin and the target not visible increasing the risk to lose the sample during trimming. Using fluorescence imaging allows to visualize a fluorescent sample inside the resin. UC Enuity makes use of the fluorescence and enables a precise automatic trimmi...
UC Enuity Ultramicrotome - Auto Alignment of block face and knife
มุมมอง 42610 หลายเดือนก่อน
Empower your team through automation! Have you ever struggled with aligning block face and knife for getting good sections on the ultramicrotome? UC Enuity takes over this task and automatically aligns block face and knife for highest sectioning quality. 00:00 - Unveiling the UC Enuity Ultramicrotome 00:24 - Loading knife and sample 00:47 - Start of the automatic alignment 01:14 - Start of sect...
UC Enuity Ultramicrotome - Trim your target using 3D-µCT data
มุมมอง 40710 หลายเดือนก่อน
Rely on UC Enuity to reach the burried target! Trimming a block face is a time-intensive task and takes a certain level of training. In addition, sometimes the sample is burried inside of the resin and the target not visible increasing the risk to loose the sample during trimming. Using a µCT allows to visualize the sample inside the resin. UC Enuity makes use of the µCT-data and enables a prec...
How to activate the confluency module for the Mateo TL microscope
มุมมอง 222ปีที่แล้ว
You can perform consistent confluency measurements with Mateo TL, our digital transmitted light microscope. It increases your confidence in the success of downstream experiments. The Confluency module can be used to estimate cell confluency, the percentage of the surface of a culture vessel that is covered by adherent cells. To activate the confluency module please follow the steps within this ...
How to use the Proveo 8 ophthalmic microscope?
มุมมอง 1.9Kปีที่แล้ว
Tutorial video on how to use the Proveo 8 ophthalmic surgical microscope. Introduction to microscope setup and basic controls. 00:00 - How to use the Proveo 8 ophthalmic microscope? 00:08 - Microscope Basic Setup 00:12 - How to activate the base brake 00:21 - How to set up your user program 00:50 - How to record videos 01:34 - How to position the integrated monitor 01:43 - How to position the o...
Documentation & Sharing with Enersight
มุมมอง 857ปีที่แล้ว
This video describes where your files are saved, how to connect your device to the network, how to share images by all operations modes (Enersight OSD, Desktop, Mobile) and save them on network folders. Learn more: fcld.ly/tl0zlet 00:00 - Documentation & Sharing 00:12 - Save files locally by Enersight Desktop, Enersight On-Screen Display and Enersight Mobile 01:07 - Save files in the network fo...
Measurement, annotations, and calibration with Enersight
มุมมอง 1.5Kปีที่แล้ว
This video describes how to: - calibrate your system - perform measurements - annotate a live image - acquire an image with measurements - save measurement or annotations as an overlay - edit previously created measurements and annotations Learn more: fcld.ly/tl0zlet 00:00 - Calibration 01:56 - Measurement 02:58 - Set up a tolerance 03:27 - Annotation 03:59 - Change the properties of drawn elem...
Comparison & Overlays with Enersight
มุมมอง 546ปีที่แล้ว
This video describes how to: - compare a live image with a reference image with a single click - acquire a single image showing a comparison view of your sample to a reference image - apply overlays to the live image - create and edit custom overlays - upload and use custom overlays - configure a footswitch or middle mouse control to toggle between custom overlays or recall the last used overla...
Image acquisition & Video recording with Enersight
มุมมอง 620ปีที่แล้ว
This video describes how to acquire image and record videos with Enersight. This video is also about the Gallery function and how to change the format and resolution of an image. Learn more: fcld.ly/tl0zlet 00:00 - Image acquisition 00:41 - Video recording 01:21 - Gallery 03:07 - Resolution and format change 03:38 - Stamp and scale bar #enersight #inspection #microscope #visualinspection #quali...
Live streaming & Camera control with Enersight
มุมมอง 795ปีที่แล้ว
This video describes how to create a live stream movie of your sample and how to control the camera via Enersight software platform. Learn more: fcld.ly/tl0zlet #enersight #inspection #microscope #visualinspection #qualitycontrol ▬ More Videos ▬▬▬▬▬▬▬▬▬▬▬▬ Subscribe @LeicaMicrosystems @LeicaMicrosystemsTutorials ▬ Follow us ▬▬▬▬▬▬▬▬▬▬▬▬ Discover us on LinkedIn: www.linkedin.com/company/leica-mi...
First steps with Enersight software platform
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First steps with Enersight software platform
Laser Microdissection for Spatial Proteomics
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Laser Microdissection for Spatial Proteomics
Consistent operation that saves you time
มุมมอง 565ปีที่แล้ว
Consistent operation that saves you time
Directly compare to references with a single click
มุมมอง 573ปีที่แล้ว
Directly compare to references with a single click
Identify defects more clearly during visual inspection
มุมมอง 777ปีที่แล้ว
Identify defects more clearly during visual inspection
Measure directly during visual inspection
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Measure directly during visual inspection
How to set up multiple fluorescent dyes for sequential imaging
มุมมอง 1.1Kปีที่แล้ว
How to set up multiple fluorescent dyes for sequential imaging
How to start and use the Software Guide and where to find the Online Help
มุมมอง 921ปีที่แล้ว
How to start and use the Software Guide and where to find the Online Help
How to set up Mica for different sample vessels and image acquisition
มุมมอง 1.2Kปีที่แล้ว
How to set up Mica for different sample vessels and image acquisition
How to extract quantitative data from your image using the AI pixel classifier
มุมมอง 1.6Kปีที่แล้ว
How to extract quantitative data from your image using the AI pixel classifier
How to optimize your acquisition using Mica’s intelligent imaging
มุมมอง 1.2Kปีที่แล้ว
How to optimize your acquisition using Mica’s intelligent imaging
Aivia Web guided tour - Your journey to AI-powered image analysis
มุมมอง 836ปีที่แล้ว
Aivia Web guided tour - Your journey to AI-powered image analysis
How to repeat image acquisition conditions with the Mateo TL microscope
มุมมอง 289ปีที่แล้ว
How to repeat image acquisition conditions with the Mateo TL microscope
Confluency measurement with the Mateo TL microscope
มุมมอง 866ปีที่แล้ว
Confluency measurement with the Mateo TL microscope
How to export images to a USB drive with the Mateo TL microscope
มุมมอง 688ปีที่แล้ว
How to export images to a USB drive with the Mateo TL microscope
How to save those 3d images? or they are just to view?
Hi there! On the bottom of the menu on the left, under "Movie Editor" it should allow you to save in different formats.
Thanks for the Explanation, Informative
how to connect it to a pc or something that send email and alert
Dear zz6761, thank you for contacting us! The easiest way to get in touch with our support team would be by filling out this form: fcld.ly/contactsupport Please let us know if you need any assistance. Best regards, your Leica Microsystems Social Media Team
You can use Sensaphone to set up an alert system. It connects to the output port at the back of the EM TP and sends an alert when the processor gives an error. You can set it up to send texts, voice and emal messages to a list of people. It will not tell you what the error is, so we also set up a webcam so if we are out of the lab we can see the screen and see what the error is and what step it is on.
But how do you create and retain calibration data after you change the magnification of the objective lens?
@burkekorol2910 Hi, thanks for your question! In short, the answer is: calibration can be done and saved under Measurements Tab -> Calibration. We can provide a more detailed answer, depending on your specific device (Enersight compatible stand or only Enersight compatible camera). Please contact us through customer service with your device details and we can provide better help that way! 👉fcld.ly/contact-gen
Ok, but like what is an easy way to re-save those individual image files as tifs? I don't know why Leica forces our images to be compiled into a .lif in the first place; it just causes unnecessary work
Hello, .lif is the default file format within LAS X, but you can easily export to tif. This video tutorial goes over how to do that: th-cam.com/video/wvQ1IC7euvs/w-d-xo.html If you have additional questions, please contact us: www.leica-microsystems.com/contact/contact-us-online/
To best support you, and reach an application specialist, please contact us here: www.leica-microsystems.com/contact/contact-us-online/ Kind Regards, Leica Microsystems Social Media Team
do you have a microscopic slide collector device?
Hi there, thank you for your interest! Please reach out to our product team for more information here: fcld.ly/sdk23ds 😊
Cool
Hello, Thanks for your helpful video! I have a question regarding time-lapse imaging using Leica! I am going to do time-lapse imaging (follow for 3 days) but I have no idea about the flow rate of water (from water pump for the objective 63X). Unfortunately, I could not find any sources to solve my problem. I wonder if you could please tell me the appropriate flow rate. Thanks!
Hi Abolfazl! Please reach out to the support team by filling out this Contact Us form: www.leica-microsystems.com/contact/contact-us-online/
veramente interessante , un bel prodotto
100% special and temporal correlation? This statement can’t be supported from philosophical, quantum mechanical and classical light theory points of view.
Hi there.. focusing could be done through microscope only or it could be done in these settings area while projecting and capturing the image?
Hi, focusing can done through both the eyepieces as well as on the monitor through the software. We recommend that, for setting up Z-stacks, a live preview in LAS X is used, in case there is any discrepancy between the focus through the eyepieces versus the camera.
Super useful information! Very cool!
Hello, I was wondering what can be done when the following message appears as you press the RFC button: Switch to z-wide and deactivate sequencer board to apply relative focus correction or activate the advance sequencer board. This message just appear and the window you have in the video is not popping up. Thanks!
Hi Raquel - go to the Project Settings Window and uncheck the Advanced Sequencer option. Then you should be able to use RFC.
How to export the huge image (navigator image)? I already reduced the tilescan size to 1% but the software still didn't allow me to export the image.
Hi Zichun - please contact your local Leica specialist or call 866.830.0735 x3 for help with exporting large files.