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hi what is the prisma website with the tool for the flow diagram?

Thank you, Victoria & Kirsten! This helps with my paper. God blesses both of you 🙏🏻

Great video. Thanknyou. Any reference for the temperature during the experiment? We're working at ~22° C

Very informative and impressive video.🤩👌 Really whole channel is a library of knowledge.📚 Hoping for more and more such informative content. Visualization of experiments or techniques gives a clearer idea about all over experiment and the equipment more than just reading in books or articles. Thank you for this wonderful content.🤝

Can this item being calibrated?

Very nice Thank you

Thanks you

Thanks for this video, so interesting

Where can I find more information on the theory and principles behind whole cell patch clamp recording? I'm recording from cultured mammalian cells transfected with recombinant receptors, and I would like to familiarize myself more with the different types of errors (e.g. voltage errors from currents that are too large), how to judge the quality of a seal based on the shape of the test pulse response, etc. as well as how the recording equipment operates (digitization, sampling rate, filtering, etc.) that applies to patch clamp recording in general.

Thank you for your detailed explanation.

Thank you for the helpful video. If you don't mind, I have a couple of questions. 1. When you say brief storage, exactly how brief would you say the solution would be good for? A week at most or 2-3 days? 2. If make the ACSF solution and my experiment is the next day, it should still be good as long as I bubble my solution 15 minutes before, correct? Just wanting to make sure.

Hi, thank you for this helpful video. Did you use cryosectioned fresh-frozen or fixed-frozen mouse brains? Many thanks

This is such a sophisticated experimental procedure. Thanks for this video.

I can't thank you enough for such detailed information.

Thanks so much for this really helpful video 🙏🏽. By any chance can you share a written protocol with exact measurements of each compound and liquid? I’d like to prepare this soon and want to make sure I am accurate.

Hi, you mentioned diluting ABC reagent in blocking buffer. Vector labs website mentions diluting in PBS. So, just wanted to double check with you on this? Great video btw. Thanks for sharing :)

Would you let me know the information for IBA1 such as vendor and cat/ref number ?

You explained how to giga seal in details and perfectly,

Hello, where do you place your carbogen gas tank? At my university they ask me for evidence to allow me to place it next to the recording setup. Where is your lab located?

excellent video. good job man

This is an absolutely fantastic video thank you so much.

What book teaches how to read voltage clamp recordings ?!

Great video and very informative! Is the rest of the video online too?

muito pika

Excellent Content ! Very Informative !

Nice content, Thanks.

This is great! Thank you!

great content

Great content! Thanks for this!

Very thorough! Including technical descriptions as well as conceptual. Thank you for this!

Looking for more information about the Christie Lab team, publications, or research techniques? Subscribe and visit our website at uvic.ca/christielab

Looking for more information about the Christie Lab team, publications, or research techniques? Subscribe and visit our website at uvic.ca/christielab

Looking for more information about the Christie Lab team, publications, or research techniques? Subscribe and visit our website at uvic.ca/christielab

Looking for more information about the Christie Lab team, publications, or research techniques? Subscribe and visit our website at uvic.ca/christielab