Video tutorial for the use of FInCH, a new Fiji plugin that we created for the scalable and automated whole-image analysis of protein expression and cell morphology (MethodsX (2024) 13: 102855).
We will cover FInCH installation, overview default and customized settings and grid creation, as well as an automatic transcription factor and cell morphology analyses for each image. FInCH can be used for any analyses involving specified color distribution and structural measurements where retaining controlling for size and shape change and retaining spatial information is important (e.g., feather coloration and distribution during growth or across individuals).
Download the program here: github.com/Lee-Cody/FInCH/
Cite the program: Lee, C.A., C. Sánchez Moreno, A.V. Badyaev. 2024. FInCH: FIJI plugin for automated and scalable whole-image analysis of protein expression and cell morphology MethodsX, 13: 102855.
Created by Cody A. Lee, Carmen Sánchez Moreno and Alexander V. Badyaev
0:00 Intro
0:10 Downloading Finch
0:30 Setting up Finch
2:03 Settings for analyzing transcription factor expression
2:25 Selecting images to analyze
2:46 Analyzing the images
3:25 Grid and threshold
3:49 Results
4:15 Performing cell morphology analysis
5:03 Options for grid
5:12 Results
5:34 Outro