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I can't say enough, THANK YOU SO VERY MUCH for this!!!!! FANTASTIC video and explanation

I can not see tSNE plot option when I open the FLOWSOM window, I don't know what I am missing. Can anyone help?

Very nice video and clearly explained the basic operation of FACSDiva. Though I got trained by our core facility, your video made me less frustrated for learning to use this complex machine and software.

This is so far the best video I have seen on FACS DIVA to set an experiment, to set controls, and to resolve different populations

Man, you deserve to earn $150 for every full video watched. I literally took a 2 hours training on Flow Cytometry yesterday ($75 an hour, paid by the lab though) from some super speed instructor who would not wait for me to take notes even and learned literally nothing. As soon as you started explaining what all those windows were, I knew your video is going to be great. This must be the most beautifully explained video for flow cytometry on the internet. Thank you man, I wasted my lab's money yesterday and I am learning from you today. I will watch your next video on compensation too.

Very nice video! This is really helpful! Thanks a lot

thank you so much for good demonstration!!! Extremely helpful!!!

Great video!

very well explained...thank you so much

Very nice video. It was quite helpful. Thanks for sharing :)

Thank you for the video. Very helpful. I have a question: I ran a cancer patient tumor infiltrating lymphocytes sample with NK cells BV786 for single color control of my compensation panel, the positive and negative signals did not separate very well. How to define the positive peak for this kind of situation? Thanks!

Thank you so much for this tutorial! Really well explained and easy to follow!

Thank you soo very much! I have been searching forba clear cut explanation that isnt just someone explaining the theory.

Thank you for this really good tutorial :)

The best video for flow cytometry experiment found ever

Thank you!!!!!

Clear explannation

Excellent demo! Extremely helpful, thanks for sharing this video!

Excellent presentation. It is clear, concise and engaging. I truly appreciate your willingness to share your expertise.

Send some traditional pigs from ur country i need to genes testing purpose

Can u tell this software name which is used by this machine

Well done, David. Sometimes it's easier just to manually check your tanks as well!!

Helpful to me

Did you perform automatic compensation before that or is it possible to set up a panel directly like that ? I suppose that automatic compensation is needed when you also need to titrate antibodies and decide voltages. By the way the a automatic process is the only way to tell the program about negative controls right ?

Awesome video! Please do more videos like this one; it's such great tutorial material. Thank you!

Thank you for being much more helpful than anyone in my lab or institution. Much appreciated 🙏

😀Very useful. Thanks,

Many Thanks

video quality is extremely poor so sad

Thank you so much! This has been really helpful :) It was easy to follow. And I like that you explained the individual parts (tsne, FlowSOM) before showing the whole process merged together.

Lovely presentation for the flow cytometry. It really helped me a lot. Many thanks.

Thank you for the video ! This is very informative!!

Thanks a lot! Very clearly explained...

Please have more videos that walk us through as beginners how we use a cell sorter like Aria in cell analysis. This is, by far, the most detailed and best tutorial video I have ever watched on this instrument. Very thorough and detailed.

Do you know where we can get a user manual for FacsAria, canto, lyric and LSR?

Please make more videos. They are very detailed and very well explained. Thank you so much.

Where do we get the user manual for this instrument?

Hi, may I know the name and background of the presenter? Thank you. This video is so valuable!

Thank you very much for this. Now I have to learn how to operate FacsCanto, FacsMelody,FacsLyric, LSR etc.

One of UC Merced's strengths besides energy materials is stem cell research. Their facility is top notch!

thanks alot for your great and practical tric🙏🏻🙏🏻, you can either put a small fixed thing instead of a spring for passing this error

Thanks for the tutorial! Very clearly explained! One question about comparing FlowSOM population frequencies. Let's say I have a control and a treatment group, with 5 samples in each group. I create a single concatenated file with all 10 samples, then separate each sample using sample ID gates, and then run FlowSOM on that whole concatenated file. Then I drag and drop the created FlowSOM population gates onto each one of the Sample ID gates. My question is: Can I then run a simple two-tailed t-test to compare the frequency of a given flowSOM population between control and treatment samples? Would that be statistically valid/"legal"?

Thanks bro. This really helped a lot.

Thank you!!! That makes sense for the beginner!

Thank you so much. It's very useful!

It is a great tutorial! Well explained, moving with good speed and presented in a very logical fashion. Thank you so much!

Thank you for such a great video! I am learning flow cytometry and this helps a lot

When you ran the compensation, did you run Low or High? And also for the cells? Thanks

Character In the video It's great, I like it a lot $$

The video sound is pretty good, beyond my imagination