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Great presentation and video! You mentioned several uncontrollable factors, such as varying diet and lifestyle, as limitations of the study. Can you suggest some solutions to these issues?

Great job! I appreciate that you mentioned the type of results you are expecting in your video. I have one question - do you homogenize or mix the soil while watering? How would that affect the results?

Great job! How long does the herbal tea linger in the mouth? How might this impact the outcomes? Are you seeking a broader, overall response from consuming herbal tea or the direct effect of herbal tea exposure in the oral cavity?

"Your study was presented very clearly. Although the data may not be representative of a larger population, it does not necessarily mean that the data will not be "reliable" (~8 min 30 sec). As long as your conclusion is specific to this study and discuss about the changes in bacteria after treatment in the test subjects

Thank you for sharing the details of your study. Are the mushrooms for each group from a single package? Do you think there might be any other forms of contamination from the environment during the packing and distribution process that could affect the reliability of your data and interpretation?

This study is very relevant because many people may frequently leave out their open packs of kimchi. At around 6 minutes and 50 seconds, it was mentioned that the different fermentation times of various batches/packs of kimchi is a limitation. So the question is, why did you choose to test different batches of kimchi? Isn't that for better sampling?

This is an interesting study. How would you connect your data to the effect of yogurt on eczema development? Do any of the test subjects have eczema? If you do obtain the expected results, such as increased S. thermophilus & L. bulgaricus and reduced S. aureus, is that enough to support the claim that yogurt can have a positive impact on eczema?

Great presentation! Did you store the samples at -20 degrees Celsius or -80 degrees Celsius before DNA extraction? Do you think that is sufficient to preserve the sample integrity during storage, or would adding stabilizers help? Have you controlled for the skin surface area that is treated or swapped between individuals?

Do you think acid rain will end up enriching for acid-tolerant lactic acid bacteria, many of which are well known probiotics?

By removing the soil sample from its natural condition, the microbiome may become less resilient to changes in pH. Given more resources and time, can you think of an experimental set-up that would allow you to assess the effect of pH on the soil microbiome outside?

When it comes to gut health, "beneficial" bacteria are commonly isolated from fermented/dairy products. Does the literature suggest that these bacteria can similarly be isolated from agricultural products (specifically mushrooms)? If not, what beneficial bacteria do you expect to find on the organic mushrooms?

Very pretty slides! With the results from Uni of Malaysia's study, did your group look out for any of the reported benefits?

Nice presentation! Am glad you pointed out how this is an unnatural/abrupt pH change and other limitations might impact the conclusions that can be drawn. A little amused that you use a sterile shovel to dig soil haha!

Nice videography - I notice you filmed from morning to night! A for effort!! I wonder if instead of drinking 1 full can, if the impact would have been more pronounced if you held a mouthful or herbal tea in your mouths for 2 minutes straight instead - but it was good that you thought about not using a straw! I also wonder if the temperature of tea (presumably most herbal teas would be taken warm/hot) would make a difference

Nice storyline but also a few lab safety lapses! Really like the effort taken to control for potential confounders. Any literature supporting use of these prebiotics for the commensals of healthy scalp?

Nice work with the news format that makes it more fun and engaging! Also a very refreshing and original choice of topic! Just wondering: do inorganic mushrooms need a lot of artificial fertilizers and pesticides to grow?

Nice presentation! Thank you for the information in 0:43 about fermentation times. I am a little surprised that Kimchi only takes 1-2d at 30deg to completely ferment! Looking fwd to see what happens if we leave it out at RT for 2d more =)

Hi Group2, very nice presentation! At 2:25, you talk about S. thermophilus and L. bulgaricus being commensals - are they naturally found on human skin? why is sample collection in a nonsterile environment a significant limitation?

Well done, group1! Interesting and engaging video that gets us all curious about the results! Just wondering, what is the rationale of swabbing after shower and not before? They may have been killed by preservatives, but wouldn't the DNA from these "probiotics" still be present at higher levels anyways? Finally, is there a better way to decide on study sample sizes instead of just following another study that has been published? Just because it is published doesn't make it right/good =)

Why was this bifidobacterium strain used and not other strains? (Bifidobacterium bifidum BGN4 and Bifidobacterium longum BORI)

It’s mentioned that there is a significant decrease in pro inflammatory genera within the probiotic group. However, the authors do not appear to have conducted any statistical analyses comparing abundance in the probiotic and placebo groups (which in some cases appear quite similar). Further, the relative abundance of these genera periodically decreases and increases regardless of treatment. Do you think the conclusion that probiotic supplementation beneficially influenced gut microbial communities is still valid?

Hello i would like to know how would the longer intervention period show better results?

There were no significant changes at the phylum level but at the genus level, what factors could explain this disparity? Could these changes have broader implications for understanding the efficacy of probiotic interventions?

Thank you for the interesting presentation! Were there any differences in S. aureus mutations between participants with mild and severe AD, if severity was considered in the study?

Nicely done presentation. It was mentioned in the presentation that the lack of variation in strains was a limitation of the study, and since wo strains that were used in the study are already well-established strains that are good probiotics, what is the value/motivation behind exploring other strains?

Helo. How does the change in bacteria abundance as mentioned in the slides relate to changes in BDNF levels?

How do the strains of bifidobacterium that are positively correlated with depression differ from those that are negatively correlated?

In the bioinformatics portion, you mentioned that Amplicon Sequence Variants (ASVs) were used for taxonomic identification. Why is that so? There are many other studies that used Operational Taxonomic Units (OTUs) (such as group1's study). What is the difference?

in the interpretation it was mentioned that candidates for psychobiotics can be derived. can you elaborate a bit on what psychobiotics are and how the strain specific nature of bifidobacteria can help to identify these candidates?

Nice video! The ending was great!! :) Do you think the gut microbiome and metabolites of ALS against healthy people would differ from the findings in different countries, even provinces?

What are your thoughts on this experiment translating to different ethnic patients clinically diagnosed with depression? How do you think their gut biota would be like? How would the results change? Good job on the presentation! Just some formatting suggestions: - take note of the speaker screen at the top right, sometimes it blocks out your words on the slides -some slides are too wordy. Might want to consider using graphics like flow charts and etc to reduce words - for results, if the figures from the paper does not add value then i suggest it can be replaced with (self-made) graphics instead??? Cause they are too small and I can’t and don’t really know where to see 😢 you can use it but perhaps would be good to highlight certain important results for viewers (box it up, highlight, pop-out box.. etc) Thank you for an informative presentation. All the best with the rest of the semester!!! 🔥👏🏼😛

what would you expect for an individual of mixed ethnicity? Would the microbiota be a mixture of her parents' ethnicity?

Thank you for your sharing and explanations of this paper! I was wondering if the group could provide more clarification on what was meant by the study limitation, "deviations from the disease phenotype (DSS) does not necessarily imply a healthier state or rescue". What would deviations from the disease phenotype mean in this case then? Could they also have different but still negative impacts on health?

Thanks for this interesting video! I found this study quite interesting as I think potential biomarkers are really needed in the context of ALS provided the large time gap between disease onset and eventual diagnosis! As ALS is often considered a rare disorder, I was wondering what you guys think can be done to incentivise researchers/companies to conduct further studies (as you described at the end) into microbiome dysbiosis as a diagnostic tool for rare diseases like ALS?