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Sartorius BIA Separations
Slovenia
เข้าร่วมเมื่อ 2 มี.ค. 2008
www.biaseparations.com/
BIA Separations is the leading developer of monolith technology and the exclusive producer of CIM® (convective interaction media) chromatographic columns for the production, purification, and analysis of large biomolecules.
Our multi-use research, production, and training facility is headquartered in Ajdovscina, Slovenia with sales and distribution offices located internationally.
Our ongoing mission is to develop and produce CIM® monolithic columns of the highest quality and provide superior research and method development services for our customers. We now offer the highly versatile PATfix™ HPLC system for producing analytical gradient separations.
BIA Separations is the leading developer of monolith technology and the exclusive producer of CIM® (convective interaction media) chromatographic columns for the production, purification, and analysis of large biomolecules.
Our multi-use research, production, and training facility is headquartered in Ajdovscina, Slovenia with sales and distribution offices located internationally.
Our ongoing mission is to develop and produce CIM® monolithic columns of the highest quality and provide superior research and method development services for our customers. We now offer the highly versatile PATfix™ HPLC system for producing analytical gradient separations.
Exploring Bacteriophage Purification with Arik Makovitzki
Bacteriophages, or phages, are viruses that infect and kill bacteria, offering a promising approach to combat antibiotic-resistant strains. In this insightful interview, Dr. Arik Makovitzki from the Israel Institute of Biological Research shares his experiences and challenges in purifying these phages, highlighting innovative solutions that have transformed his work.
มุมมอง: 49
วีดีโอ
Quality by Design in mRNA Manufacturing
มุมมอง 33914 วันที่ผ่านมา
The COVID-19 pandemic triggered an unprecedented surge in development of mRNA-based vaccines and other therapeutics, such as protein replacement therapy and cancer. mRNA is produced by a cell-free process based on in vitro transcription (IVT) reaction, a RNA-polymerase-catalyzed polycondensation of NTPs into a nascent mRNA chain guided by DNA template. mRNA production workflow is adaptable to p...
Improving the Safety of LNP-mRNA Therapeutics and Vaccines by Utilizing Monolithic Chromatography
มุมมอง 411หลายเดือนก่อน
Lipid nanoparticle (LNP) based therapeutics and vaccines have been at the forefront of the non-viral delivery since SARS-CoV-2 vaccines rolled out and proved their worth. LNP’s safety profile is a critical attribute that must be considered during its formulation and manufacturing. Major safety issues can stem from the heterogeneity of LNPs, including the presence of impurities such as empty par...
Comparability of 5 Alternative AAV9 Downstream Processes from Ultracentrifugation to Chromatography
มุมมอง 2803 หลายเดือนก่อน
Improved analytical methods are helping improve the understanding of capsid heterogeneity in adeno-associated virus (AAV) therapeutic preparations. This understanding allows the development of manufacturing processes that produce AAV therapeutics with fewer non-functional capsids. In this video, the speakers will present the results of a five-arm AAV9 comparability protocol that includes four a...
From Process Development to Manufacturing: Optimize and Scale up the AAV Enrichment Step
มุมมอง 2385 หลายเดือนก่อน
Adeno-associated viruses (AAV) have emerged as leading vectors for gene therapy applications due to their low pathogenicity and ability to mediate long-term gene expression in a wide range of tissues. In each AAV downstream process, one of the key steps (and often a bottleneck) is enrichment of full capsids. Along with scalability and capsid separation challenges, one should account for sample ...
Optimizing LNP Formulations Leveraging Monolithic Columns for Precise Insight and Improvement
มุมมอง 3026 หลายเดือนก่อน
Lipid nanoparticles (LNPs) have emerged as the foremost non-viral carriers for therapeutics and vaccines, capable of encapsulating various payloads. LNP carriers provide a significant advantage over traditional viral delivery systems, enabling modularity, speed, and better scalability. However, they pose significant challenges in terms of their purification and characterization. Post-formulatio...
Scalable chromatographic strategies for mRNA and saRNA purification from IVT
มุมมอง 3817 หลายเดือนก่อน
The potential of RNA-based therapies continues to be explored in the vaccine segment, which has seen strong development during the COVID-19 pandemic, and beyond. Besides messenger RNA (mRNA), emerging RNA modalities such as self-amplifying RNA (saRNA) and circular RNA are gaining interest for their ability to reduce dosage or expand into new therapeutic areas. However, the structure and propert...
Method Optimization & High Throughput Purification for mRNA and rAAV
มุมมอง 1988 หลายเดือนก่อน
The downstream processing of virus particles, vesicles, RNAs, plasmids and other forms of DNA, contains multiple interdependent steps, each requiring optimization for best results. In this webinar we showcased how to shorten the development time by screening multiple conditions at once, with small sample intake and process automatization. In these processes we used our newly launched CIM® monol...
How to Operate CIM Well Plates With Vacuum Manifold
มุมมอง 7999 หลายเดือนก่อน
0:00 How to Operate CIM Well Plates With Vacuum Manifold 0:56 How to Properly Store CIM Well Plates Learn how to properly store well Plates after experiments by watching the video below: th-cam.com/video/OcWcymL_jEw/w-d-xo.html Discover the most recent webinars showcasing CIM Monolithic Well Plates technology: th-cam.com/play/PLSdD7ZOG-GtLfvi1PP0ae6L0GYez60yoe.html EXPLORE OUR WELL PLATES: www....
How to Operate CIM Well Plates With Positive Pressure Manifold
มุมมอง 1.6K9 หลายเดือนก่อน
0:00 How to Operate CIM Well Plates With Positive Pressure Manifold 0:44 How to Properly Store CIM Well Plates Learn how to properly store well Plates after experiments by watching the video below: th-cam.com/video/OcWcymL_jEw/w-d-xo.html Discover the most recent webinars showcasing CIM Monolithic Well Plates technology: th-cam.com/play/PLSdD7ZOG-GtLfvi1PP0ae6L0GYez60yoe.html EXPLORE OUR WELL P...
How to Operate CIM Well Plates With Centrifuge
มุมมอง 2869 หลายเดือนก่อน
0:00 How to Operate CIM Well Plates With Centrifuge 0:54 How to Properly Store CIM Well Plates Learn how to properly store well Plates after experiments by watching the video below: th-cam.com/video/OcWcymL_jEw/w-d-xo.html Discover the most recent webinars showcasing CIM Monolithic Well Plates technology: th-cam.com/play/PLSdD7ZOG-GtLfvi1PP0ae6L0GYez60yoe.html EXPLORE OUR WELL PLATES: www.biase...
Practical Applications of CIM Monolithic Well Plates
มุมมอง 1.1K9 หลายเดือนก่อน
Chapters: 0:00 Practical Application of CIM Well Plates 0:20 First Use 0:40 How to Prepare a Plate for Operation 1:05 Cleaning and Storage Procedure Before first use read the Instruction for Use for your Product. You can download the document from our website: www.biaseparations.com/en/certificates Discover the most recent webinars showcasing CIM Monolithic Well Plates technology: th-cam.com/pl...
Maximizing mRNA Production: At-line Process Monitoring for Improved Purity and Yield
มุมมอง 46910 หลายเดือนก่อน
Speaker: Rok Sekirnik, Sartorius BIA Separations Title: Maximizing mRNA Production: At-line Process Monitoring for Improved Purity and Yield Date & Time: December 5, 2023 | 9:00 AM EDT Abstract: The talk will present case studies of leveraging the power of at-line HPLC monitoring for process development to increase purity and yield of mRNA production. Approaches to optimize plasmid purificatio...
Cost Efficient Manufacturing of Purer AAV Gene Therapy Vectors
มุมมอง 29710 หลายเดือนก่อน
Speaker: Aleš Štrancar, Sartorius BIA Separations Date & Time: Wednesday, December 13 | 11:00 AM Eastern Standard Time Abstract: The goal of AAV downstream process (DSP) is to achieve high recovery of more than 90% of full AAV capsids. For the first column step, either affinity or cation exchange chromatography is used. For the polishing anion exchange, chromatography or ultracentrifuge is empl...
Robust Manufacturing of Purer AAV Gene Therapy Vectors
มุมมอง 20410 หลายเดือนก่อน
Speaker: Aleš Štrancar, Sartorius BIA Separations Date & Time: Tuesday, Dec 12th | 12:30 PM Europe Time Abstract: The main goal of AAV downstream process (DSP) is to achieve high recovery of more than 90% full AAV capsid. For the 1st column step either affinity or cation exchange chromatography is used. For the polishing anion exchange chromatography or ultracentrifuge is employed. In this webi...
Improved Capsid Purification in AAV Manufacturing
มุมมอง 63311 หลายเดือนก่อน
Improved Capsid Purification in AAV Manufacturing
New Methods to Separate Empty, Partial, Full and Heavy AAV Capsids
มุมมอง 67511 หลายเดือนก่อน
New Methods to Separate Empty, Partial, Full and Heavy AAV Capsids
New Empty|Full AAV Method Supported With Novel CIMmultus HR Columns
มุมมอง 604ปีที่แล้ว
New Empty|Full AAV Method Supported With Novel CIMmultus HR Columns
An Innovative Analytical Platform for Evaluating Vital LNP Characteristics
มุมมอง 405ปีที่แล้ว
An Innovative Analytical Platform for Evaluating Vital LNP Characteristics
PATfix Analytical System With Multiple Detectors | At-Line Monitoring
มุมมอง 638ปีที่แล้ว
PATfix Analytical System With Multiple Detectors | At-Line Monitoring
Accelerate Your AAV Process Development With a New Wave Analytical Tool
มุมมอง 24Kปีที่แล้ว
Accelerate Your AAV Process Development With a New Wave Analytical Tool
Chromatography in mRNA Production From Analytics to Purification
มุมมอง 1.5Kปีที่แล้ว
Chromatography in mRNA Production From Analytics to Purification
Virtual Lab Tour | Viruses, Exosomes, and Virus Like Particles
มุมมอง 4.3Kปีที่แล้ว
Virtual Lab Tour | Viruses, Exosomes, and Virus Like Particles
Chromatographic Modalities, Buffer Selection and Other Considerations for Enrichment of AAV Product
มุมมอง 910ปีที่แล้ว
Chromatographic Modalities, Buffer Selection and Other Considerations for Enrichment of AAV Product
Optimize Your mRNA process from pDNA template to LNP Encapsulation With a Single Analytical Platform
มุมมอง 1.3Kปีที่แล้ว
Optimize Your mRNA process from pDNA template to LNP Encapsulation With a Single Analytical Platform
Chromatography in mRNA Production: From Analytics to Purification
มุมมอง 3.3Kปีที่แล้ว
Chromatography in mRNA Production: From Analytics to Purification
Chromatographic Tools for Isolation of Extracellular Vesicles and Process Monitoring Analytics
มุมมอง 423ปีที่แล้ว
Chromatographic Tools for Isolation of Extracellular Vesicles and Process Monitoring Analytics
Fast & Efficient rAAV Downstream Process Optimisation With CIM Monolithic 96-well plates
มุมมอง 5942 ปีที่แล้ว
Fast & Efficient rAAV Downstream Process Optimisation With CIM Monolithic 96-well plates
Chromatography in mRNA Production: From Analytics to Purification
มุมมอง 1.4K2 ปีที่แล้ว
Chromatography in mRNA Production: From Analytics to Purification
PATfix analytical platform for monitoring mRNA production process
มุมมอง 1.6K2 ปีที่แล้ว
PATfix analytical platform for monitoring mRNA production process
Yes, BUT How did you get pDNA from mRNA? was it by reverse transcription, followed by cloning the cDNA into a plasmid vector, such as pmRNAxp? and then use the plasmid DNA as templete for in vitro transcription? these are not clear from this talk!?
How did you get pDNA from mRNA? was it by reverse transcription, followed by cloning the cDNA into a plasmid vector, such as pmRNAxp? and then use the plasmid DNA as templete for in vitro transcription? these are not clear from this talk!?
Hi and thank you for your question. As shown on the slide, plasmid DNA is isolated from E. coli, then linearized using a restriction enzyme. Linearized plasmid DNA is used as a template to produce mRNA in IVT reaction. mRNA is then purified from IVT as described in the webinar. Plasmid engineering (cloning) is not a topic described in this webinar. In case of additional questions please contact us directly at: help@biaseparations.com
Oh sisters tell Parkinson a remedy too🙏
Good content is presented with minimum presentation skills!
Kindly Dr can you please send me the ppt of this webinar
Thank you for your comment. Please write to sales@biaseparations.com, so we can send you a ppt.
😱 𝚙𝚛𝚘𝚖𝚘𝚜𝚖
Jk60
Nice video for an excellent product! Making HPLC as PAT available for a routine use will have a profound effect on the industry. Keep up the great work!
I want to see the slides!
Dear Keith, you can access them here: www.biaseparations.com/en/library/seminars-webinars/1049/exosomes-new-tools-for-industrial-purification-and-process-monitoring