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you need to add something between the stacking and seperating so that your gel will be leveled. You can use isopropanol or butanol or simple DW, during the 30 - 60 mins wait time. Read Schagger protocol. Many issues.

This is a gorgeous learning resource thank you so much. I wish there were more demonstrations like this of lab protocols online.

what is the ml of sulfuric acid?

The music 🔥

Great explanation, but the bases are spelled wrong in a few images. It is Guanine, Cytosine, Thymine, and Adenine. You spelled them wrong in a few places.

How many uL are 1 ug of DNA?

that tube labelled X contain what?

Dear Mister I am quite confuse at 13:24 . Does it mean value = 0.488 must be in between 0.43 and 0. 52 of the y axis? Thank you

Which paper did u refered for this experiment

you are so cute！

Thanks for u alot.. Egypt 🇪🇬

Thank you so much, Really helpfull

Happy by seeing ❤

This comment is 50% for the algorithm, 50% because I love how well you explained everything, so good. Even liked the music... Guess I just want to let you know your time is not wasted.

how to purify protein from adherent cells ?

This video is good but the music background is so disturbing

How to prepare sample for maximum adsorption of BSA? Can you explain in detail

Thnx this video change my life for better ❤🙌🏻

Great explanation and demonstrations

Very clear procedure. Thanks a lot

you should never make a buffer in a cylinder!

AMAZING THANKS A LOT WHEN THERE IS NOONE TO HELP YOU ...EROGLU LAB IS ALWAYS TO SUPPORT..

when do you take out the rotor. If leaving it in the cylinder, it will affect the accuration.

If someone at your laboratory deliberately left 5 mL of T7 exonuclease gene 6 out at room temperature for one hour what would said to them?

Thank you, finally i understand total procedure.

such a good video

thank u very much

how much marker do you put on the gel?

can you please explain how to prepare x and how to calculate the initial concentration of x in your exemple because i didnot realy get the calculation from the dilution

Excellent. Very clear. Thank you.

Why did your gel lanes appear blue after running SDS PAGE? I mean when you dismounted the gel, the lanes looked blue and not much difference after staining?

Thanks for sharing this.

0.997 r^2 😟

can we check westernblot prepared samples with bradford? prepared sample mean samples with lemille buffer

Congratulations, I have never seen a tutorial as clear, detailed step by step and above all as complete as this one. Truly a lectio magistralis. Thank you, you are super.

In phenol sulfuric acid test for carbohydrates should we use 80% phenol or 5%phenol?? There is reference write 5% some of them write 80%??

Propably the best SEC and AKTA Pure tutorial on TH-cam! Thanks a lot

Why do you take the concentration as 0.02 and 0.04 and so on?

awesome explanatio😮

❤❤❤

Thank for video please tell me how to sample extraction for used this method thank.

Excellent, thank you!

I like video

sir I want it in written form can you provide it

I want a written rapport for this technique (materils,method, objectif and conclusion)pleaaaaaaase

Worst video I ever seen 😭

AMAZING VIDEO!! thank you so much !! :))

Really great. But may I ask what is X?

Shouldnt the curve be starting from the beginning of the two axes (point 0,0)? Because if you have no absorbance you cant have concentration. And also, isnt the standard curve a version of Lambert-Beer's law? If so, we cant have equation of y=ax + b formation, we will have the formation y=ax. And the thing i cannot understand is why we do the standard curve. Cant we just use the bradford reagent and measure the complex absorbance and after that, using Lambert-Beer's law we find the protein's concentration? Please correct me if im wrong.

thank u