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CMB LightMicroscopy
United States
เข้าร่วมเมื่อ 27 เม.ย. 2020
CMBLM - Light Microcopy core of the Cell and Molecular Biology department at St. Jude Children's Research Hospital.
Metadata extraction from file names in cell profiler
Video tutorial about how extract metadata from the input file names in Cell profiler and how to use them for name assigning and in the output files.
มุมมอง: 937
วีดีโอ
Select stack tools in ImageJ to quantify 3D data
มุมมอง 1.3K3 ปีที่แล้ว
Select stack tools in ImageJ to quantify 3D data
LAS X Navigator tutorial on Wide Field scope
มุมมอง 1194 ปีที่แล้ว
A video tutorial about how to use the LAS X Navigator feature/function on Leica SP8 wide field microscope.
Leica confocal and WF sample loading and objective cleaning
มุมมอง 3054 ปีที่แล้ว
Video tutorial about how to load a sample and clean an oil objective
Leica confocal power up and shutdown
มุมมอง 7554 ปีที่แล้ว
Video tutorial for power up and shutdown procedures on Leica SP8 confocal.
Leica Confocal Video Manual
มุมมอง 21K4 ปีที่แล้ว
Video instruction manual for Leica SP8 confocal microscope
4. Graph modifications, curve averaging, normalization.
มุมมอง 444 ปีที่แล้ว
4. Graph modifications, curve averaging, normalization.
3. Making graphs and controlling them with Window Browser.
มุมมอง 344 ปีที่แล้ว
3. Making graphs and controlling them with Window Browser.
5. Advanced capabilities: quantitation, curve fitting, presentation.
มุมมอง 324 ปีที่แล้ว
5. Advanced capabilities: quantitation, curve fitting, presentation.
Hi,i am New in imagej and Hacer a question if could you answer please? İ am trying on one image that selecting multiple rois and want to for each roi apply crop, duplicate , add gray,Binary.......and skeletonise. Making it step bu step so bored. İ tried to make a macro but i failured. Can you help me please how can i handle. Thanks
so much money for unsharp images......a DIC with photostacking gives better results
THANKYOU THANKYOU THANKYOU
Thank you.
That was very comprehensive!
这显微镜好高级啊，没用过
great video, thanks!
Very informative and important for all new Leica SP8 users! Thank you so much!
Thanks for the video. Two questions why do you set your detector range for your HyD4 (Alexa555) to 570-620 nm and dont consider 560, where the extrema emission is? And you said the optimal pixel size is half the resolution, where do I see my resolution in the manual? Thank you in advance
Discussion was not anticipated here, but short answer is that this profile general enough to accommodate various red dyes (not just AF555) and a good starting point for advanced/willing users to tweak. The wavelengths and detectors ranges are chosen for best separation of the four "standard" dyes. Also in practice AF555 is quite bright and you collect plenty of signal in this range. To give a perspective, with 50-100% gain on the HyD and 1-2% laser power you can easily saturate the detector with well stained sample. I am not sure what manual you are referring, but this reference maybe useful to look at: micro.magnet.fsu.edu/primer/java/digitalimaging/processing/samplefrequency/index.html
This video was a life saver. Thank you!
very informative. thanks for sharing