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Institutt for biovitenskap - Universitetet i Oslo (UiO)
Norway
เข้าร่วมเมื่อ 20 ม.ค. 2014
Institutt for biovitenskap (IBV), gir forskningsbasert undervisning og forskerutdanning av høy kvalitet innen et bredt spekter av biologien, - eksempelvis biokjemi, mikrobiologi, fysiologi, genetikk, evolusjon, økologi, bioinformatikk og bioteknologi. Våre kandidater får jobb i offentlig forvaltning og instituttsektoren, næringliv, skolevesen og akademia.
Episode 17 - "Interpreting Common PCR Problems from the Gel"
In the final episode, you’ll learn how to interpret various issues that may appear on your gel after PCR, with a focus on both PCR 1 and PCR 2. This episode will help you identify problems and decide on the best course of action to resolve them.
DNA Metabarcoding: Library preparation instructional video series:
Module 4 - Troubleshooting
Episode 17 - "Interpreting Common PCR Problems from the Gel"
DNA Metabarcoding: Library preparation instructional video series:
Module 4 - Troubleshooting
Episode 17 - "Interpreting Common PCR Problems from the Gel"
มุมมอง: 142
วีดีโอ
Episode 16 - "Troubleshooting Common Problems"
มุมมอง 474หลายเดือนก่อน
This episode addresses common issues encountered during the PCR process, providing practical solutions for problems like inadequate DNA yields, amplification failures, and contamination. DNA Metabarcoding: Library preparation instructional video series: Module 4 - Troubleshooting Episode 16 - "Troubleshooting Common Problems"
Episode 15 - "Equimolar Pooling of Samples for Sequencing"
มุมมอง 278หลายเดือนก่อน
In this episode, you’ll learn how to pool your PCR products equimolarly, ensuring that each sample contributes equally to the final sequencing pool. DNA Metabarcoding: Library preparation instructional video series Module 3 - PCR 2 Episode 15 - "Equimolar Pooling of Samples for Sequencing"
Episode 14 - "Quality Control and Quantification PCR 2"
มุมมอง 208หลายเดือนก่อน
Learn how to perform quality control on your PCR 2 products and quantify your products using PicoGreen quantification. This episode shows how to assess DNA concentration, ensuring your samples are ready for sequencing. DNA Metabarcoding: Library preparation instructional video series: Module 3 - PCR 2 Episode 14 - "Quality Control and Quantification PCR 2"
Episode 13 - "Operating the PCR Machine for PCR 2"
มุมมอง 450หลายเดือนก่อน
This episode covers the setup and operation of the PCR machine for the second amplification step. Viewers will see the specific program settings used to attach barcodes to the DNA samples. DNA Metabarcoding: Library preparation instructional video series: Module 3 - PCR 2 Episode 13 - "Operating the PCR Machine for PCR 2"
Episode 12 - "Pipetting the Samples for PCR 2"
มุมมอง 2.2Kหลายเดือนก่อน
Learn how to accurately pipette your DNA samples for the second round of PCR. This episode emphasizes the continued use of systematic tracking to ensure consistency and accuracy. DNA Metabarcoding: Library preparation instructional video series: Module 3 - PCR 2 Episode 12 - "Pipetting the Samples for PCR 2"
Episode 11 - "Preparing the Master Mix for PCR 2"
มุมมอง 1.4Kหลายเดือนก่อน
In this episode, you’ll see the preparation of the master mix for the second round of PCR, with a focus on the increased reagent volumes needed for indexing DNA samples. DNA Metabarcoding: Library preparation instructional video series: Module 3 - PCR 2 Episode 11 - "Preparing the Master Mix for PCR 2"
Episode 10 - "Introduction to PCR 2"
มุมมอง 1022 หลายเดือนก่อน
This episode introduces the second step of PCR, where unique barcodes are attached to DNA samples. You’ll learn about the importance of this step in preparing your samples for sequencing and how it builds on the first round of PCR. DNA Metabarcoding: Library preparation instructional video series: Module 3 - PCR 2 Episode 10 - "Introduction to PCR 2"
Episode 9 - "Magnetic bead cleaning"
มุมมอง 2362 หลายเดือนก่อน
In this episode, you’ll learn how to purify your PCR products using magnetic beads, a crucial step to remove unwanted primerdimers and ensure your DNA samples are ready for the next phase of the process. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 9 - "Magnetic bead cleaning"
Episode 8 - "Gel electrophoresis: operating the machine"
มุมมอง 1832 หลายเดือนก่อน
Following gel preparation, this episode demonstrates how to load your DNA samples into the gel and run the electrophoresis machine. You’ll see how to visualize the results, helping you assess the success of your PCR. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 8 - "Gel electrophoresis: operating the machine"
Episode 7 - "Gel electrophoresis: preparing the gel"
มุมมอง 2372 หลายเดือนก่อน
This episode introduces gel electrophoresis, focusing on preparing the agarose gel to separate DNA fragments. Viewers will learn how to create the gel mold and pour the gel for effective analysis. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 7 - "Gel electrophoresis: preparing the gel"
Episode 6 - "Operating the PCR machine for PCR 1"
มุมมอง 1442 หลายเดือนก่อน
Learn how to properly set up and operate the PCR machine for the first round of amplification. This episode covers everything about programming the thermal cycler. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 6 - "Operating the PCR machine for PCR 1"
Episode 5 - "Pipetting the samples for PCR 1: A systematic approach"
มุมมอง 1422 หลายเดือนก่อน
This episode guides you through the careful process of adding DNA samples to the PCR tubes, using a systematic method to track progress and minimize errors during pipetting. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 5 - "Pipetting the samples for PCR 1: A systematic approach"
Episode 4 - "Pipetting the master mix for PCR 1 in the UV-cabinet"
มุมมอง 3632 หลายเดือนก่อน
In this episode, you’ll see how to efficiently pipette the prepared master mix into PCR tubes within the sterilized UV cabinet. Emphasis is placed on maintaining a systematic approach to avoid contamination and ensure accuracy. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 4 - "Pipetting the master mix for PCR 1 in the UV-cabinet"
Episode 3 - "Preparing the master mix for PCR 1"
มุมมอง 1482 หลายเดือนก่อน
This episode walks you through the detailed process of preparing the master mix for the first step of PCR. You’ll learn about the importance of precise measurements and the correct order of reagent addition to ensure successful DNA amplification. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 3 - "Preparing the master mix for PCR 1"
Episode 2 - "Preparing the UV-Cabinet"
มุมมอง 2002 หลายเดือนก่อน
Episode 2 - "Preparing the UV-Cabinet"
Episode 1 - "Introduction to two-step PCR"
มุมมอง 7642 หลายเดือนก่อน
Episode 1 - "Introduction to two-step PCR"
Lage mikroskopipreparater fra ulike materialer
มุมมอง 237ปีที่แล้ว
Lage mikroskopipreparater fra ulike materialer
Del 4: Justere lysbanen Köhlers prinsipp
มุมมอง 8252 ปีที่แล้ว
Del 4: Justere lysbanen Köhlers prinsipp
Del 2: Håndtering, sittestilling og innstilling av okular
มุมมอง 1.2K2 ปีที่แล้ว
Del 2: Håndtering, sittestilling og innstilling av okular
del 1 Introduksjon til bruk av lysmikroskop
มุมมอง 2.3K2 ปีที่แล้ว
del 1 Introduksjon til bruk av lysmikroskop
Highly informative video. Please continue with more model organism lab tours.
Thank you Joseph!
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Great content. You are doing good. Will definitely come back for the next. Thumbs Up 👍
Thank you so much Ethan!