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The Learning Boosters
India
เข้าร่วมเมื่อ 22 ธ.ค. 2011
Ion torrent DNA sequencing
Hello Dear Learner,
I hope you are doing well. In this lecture video, we will focus on the Ion Torrent DNA sequencing method. This method is based on the principle of detecting hydrogen ions (H⁺) released during DNA synthesis on a template DNA strand. It falls under the category of sequencing by synthesis.
Here's an overview of the Ion Torrent DNA sequencing process:
DNA Preparation: In this step, DNA is isolated and fragmented using physical methods such as sonication. The generated fragments are then ligated with two different types of adapters.
Library Preparation: This step involves emulsion PCR, where single-stranded DNA is generated on beads.
Sequencing: In this step, each bead, carrying a DNA fragment, undergoes a customized DNA synthesis process. During this process, single nucleotides (dNTPs) are added one at a time. The addition of each nucleotide is measured by detecting the release of hydrogen ions (H⁺).
I hope you find this lecture informative and engaging. If you do, please like, share, and subscribe to our channel for more educational content.
#Genomics
#DNASequencing
#IonTorrent
#NextGenerationSequencing
#NGSTechnology
#Biotechnology
#MolecularBiology
#SequencingBySynthesis
#GenomicResearch
#EmulsionPCR
#LibraryPreparation
#Bioinformatics
#GeneticResearch
#ScienceEducation
#BiotechLecture
#EducationalContent
#ResearchTechniques
#GenomicsForBeginners
#LearnGenomics
#ScienceTutorial
#MolecularTechniques
#GeneticSequencing
#GenomeAnalysis
#DNAResearch
#InnovativeSequencing
#educationalvideos
#biotechnology
#experiment
#csirnet
#science
#emusionPCR
I hope you are doing well. In this lecture video, we will focus on the Ion Torrent DNA sequencing method. This method is based on the principle of detecting hydrogen ions (H⁺) released during DNA synthesis on a template DNA strand. It falls under the category of sequencing by synthesis.
Here's an overview of the Ion Torrent DNA sequencing process:
DNA Preparation: In this step, DNA is isolated and fragmented using physical methods such as sonication. The generated fragments are then ligated with two different types of adapters.
Library Preparation: This step involves emulsion PCR, where single-stranded DNA is generated on beads.
Sequencing: In this step, each bead, carrying a DNA fragment, undergoes a customized DNA synthesis process. During this process, single nucleotides (dNTPs) are added one at a time. The addition of each nucleotide is measured by detecting the release of hydrogen ions (H⁺).
I hope you find this lecture informative and engaging. If you do, please like, share, and subscribe to our channel for more educational content.
#Genomics
#DNASequencing
#IonTorrent
#NextGenerationSequencing
#NGSTechnology
#Biotechnology
#MolecularBiology
#SequencingBySynthesis
#GenomicResearch
#EmulsionPCR
#LibraryPreparation
#Bioinformatics
#GeneticResearch
#ScienceEducation
#BiotechLecture
#EducationalContent
#ResearchTechniques
#GenomicsForBeginners
#LearnGenomics
#ScienceTutorial
#MolecularTechniques
#GeneticSequencing
#GenomeAnalysis
#DNAResearch
#InnovativeSequencing
#educationalvideos
#biotechnology
#experiment
#csirnet
#science
#emusionPCR
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Wow crisp and clear lecture
Thanks dear...
And here I found the best explanation of illumination sequencing ❤
Thanks dear.. for your appreciation 💓 please like share and subscribe the channel
Excellent
Very easy explanation.... thank you so much sir❣️
Thank you sir
saviour fr
👏🏻👏🏻👏🏻
Waah sir ji 😍
Sir next part plz
@@itsshobi for which topic
Indeed I could understand much better because also it is explained in Hindi and that way easier to go in mind. Thanks alot Sir. Can you also explain how to perform calculation part if I have different strains of bacteria as we have different genome size how we should calculate the coverage to run all the samples in Illumina using Midoutput kit for Miniseq Illumina sequencing
PCR step occurs twice?
@@maansisrivastava541 yes as per the technique
Sir kindly deliver lectures on atomic absorption, emissions and floursescence spectroscopy
Sure...soon I will upload..thanks for your interest I hope the lecture video is helpful to you...do subscribe to the channel..♥️
Great explanation sir 👍🏼
Thanks dear...♥️
polio ki drop😂
Thank you 😊
God bless you...keep learning and stay connected... subscribe to the channel
Nice lecture sir
Waah sir ji waah
Thanks dear
best explanation ❤
Thanks for the positive comments... please like, share and subscribe..
Super explained
Thanks dear
ye to jaldi hi khatam ho gai???\
😊
nice junior entry of kid😅😅
good one....... i really like it,, contained some actual good information
Maza aaya
Ji sir smaj aaya
Kaam ki to hai ye series'
Calcium gluconate rention factor kya hoga
Dear for concept clearance please check this video th-cam.com/video/CIXkm1OjLLM/w-d-xo.html
Sir Why restriction enzyme doesn't digest methylated DNA?
Enzymes have catalytical sites for which the substrate will bind and if the substance is modified here the DNA is modified by methylation... This is one of the reasons
Keep going ❤
Thank you ♥️
Sir tomorrow is my exam and i found your videos Very helpful for me . You have got a new subscriber thank you.
All the very best for your exam....thanks for your response..GBU♥️
this video has helped me a lot! thank you so much, sir!!
Thanks for your compliment... please do subscribe to my channel and support..
Useful lecture
Thank you for your appreciation... please do like and subscribe to my channel
Thank uh sir ... 😅😊
😊
Wonder ful sir
Thank you😊
This is the simplified video i had ever get. Thank you so much sir.
Thank you so much for your kind words and feedback.. please do subscribe to the channel and support...♥️
👍
Thank You sir, Radhe Radhe Radhe-Shyam, 🙏।
Jai Ho radhe radhe... Radhey Shyam ♥️
This is the only video from where I could understand this particular topic. Thank you so much Sir🎉
Thank you for your positive response...GBU..keep learning
Good sir🎉❤
Thank you dear ❤️
Kya padate ho sir
I suppose ki aap ko samjh aya hoga topic..
❤💐❤
😊❤️
Thankyou so much sir ☺️ for this lecture
😊
sir you are a great, excellent teacher. 🔥🔥🔥🔥🔥
Thanks @hrsh ...GBU
subscribed, i wish you were in my college....10 minutes of pure explanation without changing topics and adding irrelevant information. well done sir
Thanks for your kind words ♥️
very well explained sir🙂😍 you deserve more subscribers.
Thanks for your positive comment and support ♥️
Big fan big fan padiala m buht thandi h 🎉😊
Thanks
👌👍
Nicely explained
Thank you so much 🙂
Thank you sir.. nice explanation 👌
Thank you for your positive comment .. please subscribe like.and share...
@@tlb. Ok sir ji Apne humare question ka jawab Diya esliye humne bhi subscribe kar liya. Also the reason is you have taught the topic nicely.👍👌 Jo sawal puchne pr datte hai ya confused kr dalte hai Hume wo teacher nhi pasand.
Thanks dear ..keep learning
Sif lekin sequence of gene of interest kaise pata chalega meri yahi samasya hai
Gene of interest means what gene code.for...like if you are interested in gene code for insulin you need to check the codon sequences it code for ...you can take help from bioinformatics tool..
@@tlb. Oh..so maine DNA isolate kar liya. Usme Mera gene hai So I will first have to know mi mujhe konsa gene isolate Krna and for that gene bioinformatics tools jaise ki ncbi...ense sequence Lena hai. Es sequence k aadhar par hum probe design karenge lab me. Basically gene ka Jo b base pairing arrangement ya sequence hai us hisab se bases ya nucleotides add kr k short stretch of DNA banana hai right? Ye probe hoga ...esko hybridize karana hai cDNA ya genomic DNA library me Jo cells hai uske sath. To check for binding I hope mai sahi samjhi Sahi hai?
@@tlb. Ok sir one more doubt.... So basically genomic DNA ya phir cDNA library usme se gene of interest ko catch krne k liye gene sequence pata hona jaruri hai tabhi probe attach hoga. Lekin Maine book me padha ki Yadhi gene sequence hai to we can use PCR to locate gene of interest. Aur yadi sequence nahi pata to them genomic ya cDNA library construction kr k identify Krna pdta hai. Ye kya bat Hui..doni me to gene sequence ka pata hona jaruri hai to phir PCR and libraries me farak kya raha? Please 🙏🙏🙏🙏🙏 ye jara samjha dijiye.
One of the best explanations.... Thank you 🌼
Thanks for your appreciation.. please subscribe and keep tuning ❤️